Benzoic acid, 3-amino-5-[[(2,3-dihydroxypropyl)amino]carbonyl]-2,4,6-triiodo-

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

rac-5-Amino-N-(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamic acid

EC Number:

601-093-6

Cas Number:

111453-32-8

Molecular formula:

C11 H11 I3 N2 O5

IUPAC Name:

rac-5-Amino-N-(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamic acid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
12500 mg of ZK 39.211 were dissolved in 1000 ml bidistilled water. The pH was 2.2 and was adjusted with 1 mol NaOH to 7.6. Then the further dilutions were prepared:
for the 1st: 100 mL of stock solution + 900 mL of bidist. water
for the 2nd: 10 mL of stock solution + 990 mL of bidist. water
for the 3rd: 1 mL of stock solution + 999 mL of bidist. water
for the 4th: 0.1 mL of stock solution + 999.9 mL of bidist. water
for the 5th: 0.01 mL of stock solution + 999.99 mL of bidist. Water
80 mL of the respective solution were filled into the respective test vessel.

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Schering AG
- Name and location of sewage treatment plant where inoculum was collected: The bacterium was obtained from the "Deutsche Sammlung für Mikroorganismen, Göttingen"
- Method of cultivation: kept on slant agar containing a nutrient solution at approximately 25°C. During culturing the microorganisms were transferred weekly.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test conditions

Test temperature:

room temperature

Details on test conditions:

TEST SYSTEM
- Aeration: shaking
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Test concentrations: 0.1, 1.0, 10, 100, 1000 and 10000 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

The test substance was incubated with bacteria Pseudomonas putida over aperiod of 16 h. The test concentrations were 0, 0.1, 1, 10, 100, 1000, 10000 g/L. The bacterial growth was measured on the basis of a turbidity analysis by means of a spectrophotometer. None of the concentration results differed from those of the controls. Thus, there was no indication that the testcompound affected bacterial growth.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (16 h) was higher than the highest concentration (10 g/L). Therefore, the EC10/EC50 was higher than 10 g/L.

Executive summary:

The test substance was incubated with bacteria Pseudomonas putida over a period of 16 h. The test concentrations were 0, 0.1, 1, 10, 100, 1000, 10000 g/L.The bacterial growth was measured on the basis of a turbidity analysis by means of a spectrophotometer. None of the concentration results differed from those of the controls. Thus, there was no indication that the testcompound affected bactrila growth.