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EC number: 812-548-5 | CAS number: 1621424-03-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Deviations:
- yes
- Remarks:
- Temperature range was 19.5 – 21.2 °C instead of 20.0 – 24.0 °C. As degradation of the positive control was in the normal range, this is considered as uncritical concerning the outcome of the study.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Rheinland-Pfalz, Mainz, Germany
Test material
- Reference substance name:
- {[4-({dimethyl[3-(prop-2-enamido)propyl]azaniumyl}methyl)phenyl]methyl}dimethyl[3-(prop-2-enamido)propyl]azanium dichloride
- EC Number:
- 812-548-5
- Cas Number:
- 1621424-03-0
- Molecular formula:
- C24H40Cl2N4O2
- IUPAC Name:
- {[4-({dimethyl[3-(prop-2-enamido)propyl]azaniumyl}methyl)phenyl]methyl}dimethyl[3-(prop-2-enamido)propyl]azanium dichloride
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, NW-Lachen-Speyerdorf, Germany (12. Jan. 2018)
- Preparation of inoculum for exposure: filtrated, washed with test medium (2x) and re-suspended in test medium, aerated until use
- Concentration of sludge: 25.0 mg dry matter/L (4900 mg of suspended solids/L)
- Initial cell/biomass concentration: approx. 10^7 - 10^8 cells/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD 301
- Test temperature: 19.5 - 21.2°C
- pH: 7.1 - 7.7
- pH adjusted: no
- Suspended solids concentration: 25 mg/L
TEST SYSTEM
- Culturing apparatus: 2000 mL-SCHOTT-flasks as test vessels, 100 mL scrubber flasks as absorbent vessels
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: all flasks were aerated with purified, CO2-free, moistened air to purge the system of CO2
- Measuring equipment: IC measurement using the carbon analyser TOC multi N/C 2100S, Analytik Jena, Germany
- Details of trap for CO2 and volatile organics if used: CO2 was trapped in 0.25 M NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels. The initial IC value of the 0.25 M NaOH was separately determined in each flask.
SAMPLING
- Sampling frequency: day 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29
- Sampling method: From each front scrubber flask, 10 samples were taken in order to determine the emitted CO2. The sample volume was 1 mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO2
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Other: Positive control: 2; Apparatus blank (mineral medium only): 2
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 9
- Sampling time:
- 29 d
BOD5 / COD results
- Results with reference substance:
- Degradation of the positive control was 80 % after 9 days.
Any other information on results incl. tables
Table 1: Net IC-values in mg/L of front scrubber flasks
Day |
Blank Control 1 |
Blank Control 2 |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
0 |
2.3 |
2.6 |
2.4 |
2.6 |
2.0 |
2.0 |
2.1 |
2.1 |
2 |
2.7 |
17.1 |
8.7 |
10.0 |
14.6 |
14.5 |
2.5 |
4.6 |
4 |
4.0 |
28.2 |
25.8 |
84.3 |
24.1 |
24.5 |
4.1 |
82.1 |
7 |
6.9 |
38.2 |
199.1 |
201.3 |
34.4 |
36.4 |
3.7 |
159.5 |
9 |
11.4 |
45.2 |
279.9 |
278.7 |
42.6 |
45.4 |
3.2 |
263.6 |
11 |
13.9 |
49.2 |
307.6 |
324.3 |
47.0 |
50.3 |
6.1 |
317.0 |
14 |
18.2 |
52.2 |
339.1 |
342.9 |
49.7 |
56.7 |
3.2 |
333.4 |
18 |
13.5 |
49.4 |
339.4 |
345.5 |
56.5 |
64.7 |
2.2 |
351.2 |
23 |
22.6 |
57.4 |
374.7 |
369.4 |
61.4 |
69.2 |
2.0 |
356.5 |
29 |
23.8 |
60.9 |
374.8 |
375.9 |
63.9 |
77.5 |
1.1 |
367.9 |
Table 2: Net IC-values in mg/L of back scrubber flasks
Day |
Blank Control 1 |
Blank Control 2 |
Positive Control 1 |
Positive Control 2 |
Test 1 |
Test 2 |
Abiotic Control |
Toxicity Control |
0 |
2.3 |
2.3 |
2.2 |
2.0 |
2.0 |
2.2 |
2.0 |
2.2 |
29 |
2.2 |
2.6 |
2.4 |
2.8 |
4.1 |
2.1 |
2.1 |
2.5 |
Table 3: Degradation values in %
Day |
Positive Control 1 |
Positive Control 2 |
Positive Control Mean |
Test 1 |
Test 2 |
Test Mean |
Abiotic Control |
Toxicity Control |
2 |
-0.4 |
0.0 |
-0.2 |
1.7 |
1.7 |
1.7 |
0.1 |
-0.8 |
4 |
3.2 |
22.2 |
12.7 |
2.8 |
2.9 |
2.8 |
0.6 |
10.8 |
7 |
57.1 |
57.7 |
57.4 |
3.9 |
4.6 |
4.3 |
0.5 |
22.2 |
9 |
80.5 |
80.1 |
80.3 |
4.7 |
5.6 |
5.1 |
0.3 |
37.7 |
11 |
87.4 |
92.6 |
90.0 |
5.0 |
6.1 |
5.5 |
1.2 |
45.2 |
14 |
95.2 |
96.3 |
95.8 |
4.7 |
6.8 |
5.7 |
0.3 |
46.7 |
18 |
95.4 |
97.3 |
96.4 |
7.8 |
10.4 |
9.1 |
0.0 |
49.6 |
23 |
102.6 |
101.0 |
101.8 |
6.6 |
9.0 |
7.8 |
0.0 |
48.5 |
29 |
100.8 |
101.3 |
101.1 |
7.3 |
10.7 |
9.0 |
-0.3 |
49.4 |
Table 4: Validity criteria for OECD 301B
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
3.4% |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
Pass level reached on day 9 |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
46.7% |
yes |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC. |
0% |
yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
8.9 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- under test conditions no biodegradation observed
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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