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EC number: 816-146-0 | CAS number: 1016788-34-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January to February 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted April 13, 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Commission Regulation (EC) No 440/2008, C.2., 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling: Duplicate samples from the freshly prepared test media of all test concentrations and the controls were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all
test concentrations and the controls were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.
Additional samples of the control were taken at test start and test end.
Analyses: The concentrations of the test item GenoWhite were analysed in the duplicate test media samples from all test concentrations, and in the duplicate control samples, from both sampling times (0 and
48 hours). - Vehicle:
- yes
- Remarks:
- Test item dissolved in test water.
- Details on test solutions:
- The test medium of the highest test concentration of nominal 100 mg test item/L was prepared by dissolving 60 mg test item into 600 mL test water by intense stirring for 15 minutes. Adequate volumes of
this test medium were diluted with test water to prepare the test media.
The test media were prepared just before introduction of the Daphnia (= start of the test). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna (Straus), clone5; 2.75 to 18.75 hours old.
Sex: Female.
Origin: Daphnia introduced in the test were taken from ibacon's in-house laboratory culture.
Breeding Conditions: The Daphnia were bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental Daphnia was performed in Elendt M4 medium (see 6.5). The test organisms were not first brood progeny. The Daphnia in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the
laboratories of ibacon.
Reference Item: For the evaluation of the quality of the Daphnia clone and the consistency of the experimental conditions, the reference item potassium dichromate is tested at least twice a year to demonstrate
satisfactory test conditions. The results of the most recent reference test are included in Table 10.
Acclimatisation: Was not necessary, since the test was performed in the same medium as the culturing (see 6.5). - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Reconstituted Water (Elendt "M4")
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.5 mmol/L as CaCO3
- Test temperature:
- 20-19 ºC
- pH:
- 7.8 to 8.0
- Dissolved oxygen:
- 9.8 to 10.0 mg/L at test start
9.6 to 9.9 mg/L at test end - Salinity:
- Alcalinity: 0.9 mmol/L
- Nominal and measured concentrations:
- At the start of the test 94% of the nominal test concentrations were found (average of all test concentrations). After 48 hours test duration, 92% of the nominal value was determined (average of all test concentrations). During the test the daphnids were exposed to a mean of 93% of nominal. Therefore, all reported results refer to nominal concentrations.
- Details on test conditions:
- Test Units
Type and Size: Glass beakers of 100 mL volume containing approximately 60 mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions.
Identification: Each test unit was uniquely identified with serial number, treatment and replicate number.
Test Conditions
Test Environment: Controlled environment room
Measurement of pH, Dissolved Oxygen and Water Temperature: The water temperature, pH-values and dissolved oxygen concentrations were determined at test start and test end in each treatment group.
Water Temperature: 20 to 20 °C at test start; 19 to 19 °C at test end
pH-Values: 7.8 to 8.0 at test start; 7.8 to 7.9 at test end; and thus the pH-value did not vary by more than 1.5 units
Dissolved Oxygen Concentration: 9.8 to 10.0 mg/L at test start;9.6 to 9.9 mg/L at test end (see Table 6)
Light Regime: 16 h light : 8 h dark
Light Intensity: The light intensity was 210 to 670 lux (measured once during the test).
Recording: Test conditions were recorded with suitable instruments and documented in the raw data. - Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Validity Criteria of the Study
Control Immobilisation Rate: Was 0 % and furthermore no daphnid showed signs of disease or stress; thus the validity criterion was met.
Dissolved Oxygen Concentration: Was ≥ 9.6 mg O2/L in the control and test vessels at the end of the test; thus validity criterion was met.
Biological test results: After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the highest test item concentration of 100 mg test item/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic effect of the test item to Daphnia magna was assessed in a static dose-response test. The 48-hour NOEC was determined to be ≥ 100 mg test item/L.
The 48-hour LOEC was determined to be > 100 mg test item/L and the 48-hour EC50 value was determined to be >100 mg test item/L. - Executive summary:
The toxic effect of the test item to Daphnia magna was assessed in a static dose-response test (OECD 202: "Daphnia sp., Acute Immobilisation Test").
After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the highest test item concentration of 100 mg test item/L.
The 48-hour NOEC was determined to be ≥ 100 mg test item/L.
The 48-hour LOEC was determined to be > 100 mg test item/L and the 48-hour EC50 value was determined to be >100 mg test item/L.
Reference
Description of key information
The toxic effect of the test item to Daphnia magna was assessed in a static dose-response test (OECD 202: "Daphnia sp. Acute Immobilisation Test").
The 48-hour NOEC was determined to be ≥ 100 mg test item/L.
The 48-hour LOEC was determined to be > 100 mg test item/L and the 48-hour EC50 value was determined to be >100 mg test item/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 ng/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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