Acetoacetamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 October 2017 - 29 November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Analytical samples taken at 0 hours (initial value) and 48 hours from aged test solutions were analysed from control and the highest test item
concentration of 100 mg/L.

Vehicle:

no

Details on test solutions:

The necessary amount of test item for preparing the stock solution S1 was weighed on a weighing scoop and transferred to a volumetric flask.
Test medium was added up to the bench mark and the solution was homogenised by shaking. Afterwards the solution was clear and
transparent. Lower test solutions were prepared by dilution of the appropriate solution with test
medium. ~ 50 mL of the prepared solutions were transferred to each test vessel.
Concentration: 100 mg/L, 45.5, 20.7, 9.39, 4.27, 0 mg/L

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus, Clone V, max. 24 hours old

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

13°dH (232 mg/L CaCO3)

Test temperature:

19.8 ± 0.3 °C

pH:

7.92 ± 0.08 (Std. Dev.)

Dissolved oxygen:

8.9 ± 0.3 mg/L (Std. Dev.)

Nominal and measured concentrations:

The initial measured content of acetoacetamide was 97 % of nominal and the aged measured content was 95 % of nominal.

Details on test conditions:

Light intensity was ~200 lux at test start
Exposure to light: 16 hours photoperiod /8 hours darkness daily
Feeding: none
Test vessels: four 100 mL glass beakers per concentration, each filled with ~ 50 mL, one additional replicate for pysico -
chemical measurements without test organisms
Loading: ~ 10 mL of test solution for each animal
Aeration: none
Number of animals: 20 per concentration in 4 replicates of 5

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

After 24 hours and 48 hours of exposure no immobilisations was observed in the control and up to and including 20.7 mg/L and at the highest test item
concentration of 100 mg/L. No immobilisation higher than the allowed control immobilisation was observed at 45.5 mg/L.
No behavioural changes could be observed.

Reported statistics and error estimates:

The NOEC was established based on the highest concentration at which the immobilisation is not higher than the allowed control immobilisation
(<=10 % immobilisation). The EC50 was not statistically determined. The EC50 and NOEC-values of daphnids exposed to the test item evaluated
using nominal concentrations.

Validity criteria fulfilled:

yes

Conclusions:

According to the results of the test, the EC50 (48 h) was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).

Executive summary:

A short-term toxicity study to daphnia magna was performed with the test item according to OECD 202 and GLP in year 2017.

According to the results of the test, the EC50 (48 h) was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).

Description of key information

A short-term toxicity study to daphnia magna was performed with the test item according to OECD 202 and GLP in year 2017.

According to the results of the test, the EC50 (48 h) was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

EC 50 > 100 mg/L

NOEC 100 mg/L