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EC number: 209-057-8 | CAS number: 554-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP condition
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2,4-dichloroaniline
- EC Number:
- 209-057-8
- EC Name:
- 2,4-dichloroaniline
- Cas Number:
- 554-00-7
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 2,4-dichloroaniline
- Test material form:
- other: brown-red flakes
- Details on test material:
- - Name of test material (as cited in study report): 2,4-dichloroaniline
- Physical state: solid
- Analytical purity: 99%
- Impurities (identity and concentrations): 2,6-dichloroanilin; monochloranilin (p-chloranilin)
- Lot/batch No.: GAEC 43
- Storage condition of test material: at the dark at 22 °C
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 7 weeks (males); females: 8 weeks
- Weight at study initiation:
males: ca 181.1 g; females: ca.175.1 g
- Fasting period before study: 16 h;
- Fasting after test substance administration: 4 h
- Housing: in groups of 5
- Diet e.g. ad libitum
- Water e.g. ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22
- Humidity (%): 50
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: aequous potato starch
- Details on oral exposure:
- single oral application by gavage as 2 % aequous potato starch
- Doses:
- 2000, 2500, 3150, and 4000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- other: not applicable
- Details on study design:
- Groups of 5 males and 5 females received different doses as single oral application by gavage as 2 % aequous potato starch and observed for 14 days mortality and symtoms were recorded; Body weight determination once before treatment, once during observation period and once at termination of the study. Necropsy and gross pathological examination of all animals
- Statistics:
- The LD 50, the 95% confidence level and the equation of Probit linear slope were calculated from the encountered rate of lethality using the Probit analysis.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 110 mg/kg bw
- 95% CL:
- 2 620 - 3 870
- Remarks on result:
- other: cyanosis (up to 4 days post application), irregular respiration, blood-coloured secretions of noses and eyes, clonic convulsions and miosis, nonspecific symptoms: effects on the musculoskeletal system. All symptoms disappeared within 6 days.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 2 830 mg/kg bw
- 95% CL:
- >= 1 860 - <= 3 940
- Remarks on result:
- other: calculated from mrtality data
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- ca. 3 330 mg/kg bw
- 95% CL:
- >= 2 590 - <= 5 180
- Remarks on result:
- other: calculated from mortality data
- Mortality:
- Lethality occurred until 3 days after application:
males and females:
2000 mg/kg bw: 0/5 and 1/5
2500 mg/kg bw: 1/5 and 2/5
3150 mg/kg bw: 3/5 and 2/5
4000 mg/kg bw: 3/5 and 5/5 - Clinical signs:
- other: The poisoning was characterized by nonspecific symptoms: effects on the musculoskeletal system and respiration , cyanosis (up to 4 days post application), blood-coloured secretions of noses and eyes, clonic convulsions and miosis. All symptoms were reve
- Gross pathology:
- death males and females:
The macroscopic findings showed discoloration of dead animals´ adrenal glands, bleeding in the small intestine, congestion of the lungs and bulging bladders.
surviving annimals:
The section of the surviving animals showed no macroscopically visible changes
Applicant's summary and conclusion
- Executive summary:
2,4 -dichloroaniline was tested for acute oral toxicity according to OECD TG 401 under GLP conditions. Groups of male and female Wistar rats received 2000 -4000 mg/kg bw as single application suspended in aequous potato starch and were observed for 14 days for mortality, clinical symptoms and body weight development. After termination of the observation period surving animals were sacrificed and all animals were examined macroscopically.
.Animals showed cyanosis (up to 4 days post application), irregular respiration, blood-coloured secretions of noses and eyes, clonic convulsions and miosis, and nonspecific symptoms of the musculoskeletal system. All symptoms disappeared within 6 days. Body weight development was not affected by treatment. Death occurred within 3 days after application resulting in a LD50 value of 3110 mg/kg bw for male and female rats.
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