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EC number: 209-057-8 | CAS number: 554-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity tests: V. Results from the testing of 311 chemicals
- Author:
- Zeiger E, Anderson B, Haworth S, Lawlor T, and Mortemals K
- Year:
- 1 992
- Bibliographic source:
- Environmental and Molecular Mutagenesis. Volume 19, Supplement 21:2-141
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- No GLP study. No E.coli strain tested. Only 4 strains tested
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,4-dichloroaniline
- EC Number:
- 209-057-8
- EC Name:
- 2,4-dichloroaniline
- Cas Number:
- 554-00-7
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 2,4-dichloroaniline
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in publication): 2,4-dichloroaniline
- Analytical purity: >99%
Constituent 1
Method
- Target gene:
- his
Species / strain
- Species / strain / cell type:
- other: S.thyphimurium TA100, TA1535, TA1537, TA97, TA98
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- microsomal enzyme reaction mix (S9-mix) from hamster and rat liver induced with Aroclor 1254. RLI (Aroclor 1254-induced rat liver S-9); HLI (Aroclor 1254-induced hamster liver S9)
- Test concentrations with justification for top dose:
- 0, 3.3, 10.0, 33.0, 100.0, 200.0, 333.0, 500.0, 666.0 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- mitomycin C
- other: 4-nitro-o-phenylene diamine, methylmethane sulphonate, 2-aminoanthracene
- Details on test system and experimental conditions:
- pre-incubation protocoll: at least 5 concentrations of each chemical were tested in triplicate
- Evaluation criteria:
- Individual trials were judged
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: S.thyphimurium TA1535, TA1537, TA97, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at doses higher then 333.0
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Remarks:
- with S9 (5% HLI; 10% HLI, first and second trial)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- with S9 (30% HLI first and fourth trial)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 666 µg/PLATE
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- ambiguous
- Remarks:
- with S9 (30% HLI second and third trial)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 333 µg/PLATE for the second trial
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Remarks:
- with S9 (10% RLI and 30% RLI second trial )
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 333 µg/PLATE
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- ambiguous
- Remarks:
- with S9 (30% RLI first trial )
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 333 µg/PLATE
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Executive summary:
2,4 -Dichloroaniline was tested for induction of point mutations in bacteria up to 666 µg/plate using the Ames test and Salmonella typhimurium strains TA97, TA98, TA100, TA1535, TA1537 in the absence and in the presence of metabolic activation systems derived from rat and hamster livers.
2,4 -Dichloroaniline did not induce increased numbers of revertants in Salmonella typhimurium TA 1537, TA 1535, TA97 and TA 98 neither in the presence nor in the absence of a metabolic activation system. In Samonella typhimurium TA100 in the presence of 30% hamster liver S9 -mix or 30 % rat liver S9 -mix questionable or weak positive results were obtained but not in the presence of 10 % hamster liver or 10 % rat liver S9 -mix and in absence of metabolic activation systems.
Thus, based on the results obtained, 2,4 -dichloroaniline is evaluated as weak mutagen in the Ames test under the applied conditions .
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