Nitrobenzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1990

Reference 2

Reference Type:

secondary source

Title:

European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3

Author:

European Chemicals Bureau

Year:

2007

Bibliographic source:

3rd Priority List, Volume: 77

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Nitrobenzene

EC Number:

202-716-0

EC Name:

Nitrobenzene

Cas Number:

98-95-3

Molecular formula:

C6H5NO2

IUPAC Name:

nitrobenzene

Details on test material:

- Name of test material (as cited in study report): Nitrobenzol
- Analytical purity: no data available
- Impurities (identity and concentrations): no data available

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: GC

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution for every concentration
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): max. 158 mg/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc):

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Doller Zierfischzüchterei, Finnentrop-Serkenrode, Germany
- Age at study initiation (mean and range, SD): > 12 d
- Length at study initiation (length definition, mean, range and SD): 2.0 +/- 1 cm

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test?): yes
- Type and amount of food: fed daily with TetraMin

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

23° +/- 1°C

pH:

8.15 +/- 0.2

Nominal and measured concentrations:

Conc. nom.: 240.7, 120.4, 60.2, 30.1 and 15.04 mg/L
Conc. analyt.: 98, 89.9, 40.2, 26.6 and 9.9 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 11 l
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): dosing of test substance by use of a perfusor
- Renewal rate of test solution (frequency/flow rate): 6 times per day
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

92 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

44 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

98 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)