Glycerol, ethoxylated

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Comparable to guideline study OECD 403 (adopted 1981, IHT). Methodological deficiencies: no analytical determination of test atmosphere concentration; test substance purity not specified; limited documentation. Only conclusion on saturated vapor athmosphere possible.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

adopted 1981; Inhalation Hazard Test

Deviations:

yes

Remarks:

no analytical verification of test substance concentration.

Principles of method if other than guideline:

According to Smyth H.F. et al.: Am. Ind. Hyg. Ass. J., 23, 95-107; comparable to the inhalation hazard test in the Annex of the OECD test guideline 403It demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of a test substance at 20 °C. 12 rats were exposed to the vapors generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 7 h. No analytical determination of the atmosphere concentrations was performed. The nominal concentration was calculated as quotient of the amount of test substance weight loss during exposure, and the amount of air used during exposure. Group-wise documentation of clinical signs was performed over the 14 days study period. Body weight of groups was determined before the start of the study and at the end of the observation period. The exposure time not causing lethality was usually tested twice.

GLP compliance:

no

Test type:

other: Inhalation Hazard Test

Test material

Test material information

Constituent 1

Reference substance name:

Glycerol, ethoxylated

EC Number:

500-075-4

EC Name:

Glycerol, ethoxylated

Cas Number:

31694-55-0

Molecular formula:

(C2 H4 O)n (C2 H4 O)n (C2 H4 O)n C3 H8 O3 3n =>1-<6.5 mol EO

IUPAC Name:

Alkoxylation reaction product of glycerin as starter and ethylene oxyde (EO) as monomer

Details on test material:

- Name of test material (as cited in study report): Schmiermittel LK 2200
- pH: 6.5 - 7.5
- Purity: 'technical' purity

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: male mean 196 g; female mean: 178 g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

7 h

Concentrations:

Experiment 1: 0.178 mg/L nominal (calculated as quotient of the amount of test substance weight loss during exposure)
Experiment 2: 0,143 mg/L nominal (calculated as quotient of the amount of test substance weight loss during exposure)

No. of animals per sex per dose:

6 animals (males and females) per experiment (total of 12 animals)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: at the beginning and at the end of the study
- Frequency of observations: several times at the application day and thereafter once each working day
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology

Statistics:

none

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: IHT

Exp. duration:

7 h

Remarks on result:

other: no mortality occurred

Mortality:

No mortality occurred

Clinical signs:

other: Nothing abnormal detected

Body weight:

At the end of the study: females mean: 266 g; males mean: 200 g

Gross pathology:

Nothing abnormal detected

Other findings:

None