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EC number: 268-610-1 | CAS number: 68131-13-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-04-23 to 2012-06-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP, no deficiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Naphthenic acids, reaction products with diethylenetriamine
- EC Number:
- 268-610-1
- EC Name:
- Naphthenic acids, reaction products with diethylenetriamine
- Cas Number:
- 68131-13-5
- Molecular formula:
- not applicable
- IUPAC Name:
- Naphthenic acids, reaction products with diethylenetriamine
- Details on test material:
- - Name of test material (as cited in study report): Napthenic acids, reaction products with diethylenetriamine
- Physical state: Liquid, brown viscous
- Analytical purity: 100 % (UVCB)
- Purity test date: 2011-05-26
- Lot/batch No.: ESD0011831
- Expiration date of the lot/batch: 04/2013
- Stability under test conditions: Not applicable
- Storage condition of test material: Room temperature, protected from light, in original container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Directly weighing of the stock solution, 30 min ultrasound treatment at room temperature
- Eluate: Synthetic waste water acc. to OECD 209
- Differential loading: 10 - 32 - 100 - 320 - 1000 mg/L (factor 3.2) (first study)
3.2 – 10 – 32 mg/L (factor 3.2) (second study)
- Controls: Triplicates at the beginning of the test + triplicates at the end of the test
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at Hildesheim (Municipal sewage treatment plant of 31137 Hildesheim, Germany)
- Pretreatment: Washed twice with autoclaved tap water and an adjustment to a dry weight concentration of 3.0 g/L +/- 10 % was not necessary. The sludge was used within 24 h after sampling
- Dry sludge concentration: 3.14 g/L (first study); 2.73 g/L (second study)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 21.7 °C (first study) and 21.4 °C (second study)
- pH:
- pH-value of the activated sludge 7.91 first 7.26 second
pH-value of the synthetic waste water 7.56 first 7.44 second - Salinity:
- Not determined
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Composition of test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL demineralised water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter immediately after transferring the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flatbed recorder.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: None, limit test concentration
- Range finding study
Inhibition of Respiration (Preliminary Test 1,2 and 3)
Nominal Test Item Concentration
[mg/L] Mean Inhibition
[%]
1st PT 2nd PT 3rd PT
10 10 - -6
100 7 - 54
1000 13 671)
862) 88
Inhibition of Respiration (Preliminary Test 4)
Nominal Test Item Concentration
[mg/L] Mean Inhibition[%]
4th PT
Stirring Stock solution A Stock solution B
500 73 90* 90* - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 117 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI 103-141 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 54.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI 40-70 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: n.a.
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 94.0 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test were 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
The EC-values of the test item were calculated by sigmoidal dose-response regression and the EC50-value of the reference item by linear regression using software GraphPad Prism, GRAPHPAD SOFTWARE, INC. Calculation of the confidence intervals for the EC50-values was carried out using standard procedures.
Any other information on results incl. tables
From the oxygen depletion the oxygen uptake rates were calculated in mg O2/L×h, additionally the specific respiration rates based on the dry sludge concentration were calculated in mg O2/g×h.
The inhibition of the oxygen uptake rates of the test and reference item concentrations in % was calculated in comparison with the oxygen uptake rates of the control.
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration of the Control and Reference Item Concentrations(first study)
Reference Item Concentration |
|
Oxygen Uptake Rate |
Specific Respiration Rate |
|
Mean Inhibition |
Control |
1 |
36.8 |
23.4 |
– |
– |
2 |
36.8 |
23.4 |
|||
3 |
34.8 |
22.2 |
|||
4 |
28.4 |
18.1 |
|||
5 |
29.2 |
18.6 |
|||
6 |
29.2 |
18.6 |
|||
58 |
1 |
22.0 |
32 |
34 |
|
2 |
21.6 |
34 |
|||
3 |
21.2 |
35 |
|||
100 |
1 |
14.4 |
56 |
55 |
|
2 |
14.4 |
56 |
|||
3 |
14.8 |
54 |
|||
180 |
1 |
6.4 |
80 |
80 |
|
2 |
6.4 |
80 |
|||
3 |
6.8 |
79 |
Oxygen Uptake Rates, Specific
Respiration Rates and Inhibition of the Respiration of
the Control and Reference Item Concentrations (second study)
Reference Item Concentration |
|
Oxygen Uptake Rate |
Specific Respiration Rate |
|
Mean Inhibition |
Control |
1 |
24.4 |
17.8 |
– |
– |
2 |
23.6 |
17.2 |
|||
3 |
24.0 |
17.5 |
|||
4 |
20.8 |
15.2 |
|||
5 |
22.0 |
16.1 |
|||
6 |
20.0 |
14.6 |
|||
58 |
1 |
16.8 |
25 |
26 |
|
2 |
17.6 |
22 |
|||
3 |
15.6 |
31 |
|||
100 |
1 |
10.0 |
56 |
54 |
|
2 |
10.8 |
52 |
|||
3 |
10.4 |
54 |
|||
180 |
1 |
4.4 |
80 |
81 |
|
2 |
4.0 |
82 |
|||
3 |
4.0 |
82 |
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration of the Test Item Concentrations and solvent controls (first study)
|
|
Oxygen Uptake Rate |
|
Mean Inhibition |
*10 |
1 |
23.6 |
27 |
25 |
2 |
22.4 |
31 |
||
3 |
23.6 |
27 |
||
4 |
26.0 |
20 |
||
5 |
26.8 |
18 |
||
32 |
1 |
28.8 |
11 |
-2 |
2 |
31.6 |
3 |
||
3 |
32.8 |
-1 |
||
4 |
36.0 |
-11 |
||
5 |
35.6 |
-10 |
||
100 |
1 |
16.4 |
50 |
41 |
2 |
21.6 |
34 |
||
3 |
14.4 |
56 |
||
4 |
25.2 |
22 |
||
5 |
19.2 |
41 |
||
320 |
1 |
4.0 |
88 |
89 |
2 |
3.6 |
89 |
||
3 |
3.2 |
90 |
||
4 |
3.6 |
89 |
||
5 |
4.0 |
88 |
||
1000 |
1 |
1.6 |
95 |
95 |
2 |
1.6 |
95 |
||
3 |
1.6 |
95 |
||
4 |
1.2 |
96 |
||
5 |
1.6 |
95 |
||
Solvent Control 0.64 mL |
1 |
38.4 |
-18 |
-11 |
2 |
35.6 |
-10 |
||
3 |
34.0 |
-5 |
||
Solvent Control 2 mL |
1 |
21.6 |
34 |
32 |
2 |
22.4 |
31 |
||
3 |
22.0 |
32 |
Oxygen Uptake Rates, Specific Respiration Rates and
Respiration of the Test Item Concentrations and solvent controls (second study)
|
|
Oxygen Uptake Rate |
|
Mean Inhibition |
3.2 |
1 |
28.4 |
-26 |
-28 |
2 |
28.4 |
-26 |
||
3 |
29.2 |
-30 |
||
4 |
29.2 |
-30 |
||
5 |
28.4 |
-26 |
||
10 |
1 |
26.4 |
-17 |
-9 |
2 |
24.8 |
-10 |
||
3 |
23.6 |
-5 |
||
4 |
23.6 |
-5 |
||
5 |
24.4 |
-8 |
||
32 |
1 |
28.4 |
-26 |
-23 |
2 |
27.2 |
-21 |
||
3 |
27.2 |
-21 |
||
4 |
28.4 |
-26 |
||
5 |
27.2 |
-21 |
||
Solvent Control 0.64 mL |
1 |
28.4 |
-26 |
-23 |
2 |
27.6 |
-23 |
||
3 |
27.2 |
-21 |
||
Solvent Control 2 mL |
1 |
18.8 |
16 |
20 |
2 |
17.6 |
22 |
||
3 |
17.6 |
22 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item is 32 mg/L, the EC10 54.1 mg/L and the EC50 117 mg/L for activated sludge of a municipal sewage treatment plant.
- Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Naphthenic acids, reaction products with diethylenetriamine (batch number: ESD0011831) from 2012-04-23 to 2012-06-19, with the experimental phases on 2012-04-24 and 2012-06-19 at Dr.U.Noack-Laboratorien, D-31157 Sarstedt, Germany. Test system was activated sludge of the municipal treatment plant of D-31137 Hildesheim. The test was carried out under static conditions with the nominal test item concentrations 10 – 32 – 100 – 320 – 1000 mg/L (factor: 3.2). A supplementary study with the concentrations 3.2 – 10 – 32 (factor: 3.2) was carried out to support the NOEC assessment. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -2 % to 95 % in the first and -28 % to -9 % in the second study.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-values for the reference item were 87.1 mg/L and 94.0 mg/L.
EC-Values and NOEC of Naphthenic acids, reaction products with diethylenetriamine
Effect values
Confidence interval
[mg/L]
P = 95 [%]
[mg/L]
NOEC
32
n.d.
EC10
54.1
40 – 70
EC20
69.2
54 – 83
EC50
117
103 – 141
EC80
218
160 – 308
n.d.= not determinable
The NOEC of the test item is 32 mg/L, the EC10 54.1 mg/L and the EC50 117 mg/L
for activated sludge of a municipal sewage treatment plant.
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