Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-821-4 | CAS number: 52298-44-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-07-30 to 2009-08-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline conform study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-amino-2,5-dimethoxy-N-phenylbenzenesulphonamide
- EC Number:
- 257-821-4
- EC Name:
- 4-amino-2,5-dimethoxy-N-phenylbenzenesulphonamide
- Cas Number:
- 52298-44-9
- Molecular formula:
- C14H16N2O4S
- IUPAC Name:
- 4-amino-2,5-dimethoxy-N-phenylbenzene-1-sulfonamide
- Details on test material:
- - Name of test material (as cited in study report): Chinonbasesulfanilid, trocken
- Molecular formula: C14H16N2O4S
- Molecular weight : 308.36 g/mol
- Physical state: Solid, grey powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatment plant, D-31137 Hildesheim, Germany
- Pretreatment:
- Concentration of sludge: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was filled up with mineral salts medium and was maintained in an aerobic condition by aeration for four hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition by aeration with CO2 free air for 5 days- 10 mL/L were used to initiate inoculation.
- Initial cell/biomass concentration:2.1 x E9 CFU/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10.6 other: mg C/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301 B / CO2 Evolution Test
- Test temperature: 22 +/- 2°C
- pH adjusted: no
- Continuous darkness: no, low light conditions
TEST SYSTEM
- Culturing apparatus: 5000 mL brown glass flasks
- Number of culture flasks/concentration: 1 for the reference item, 1 for the test and reference item, 2 for the control, 2 for the test item
- Method used to create aerobic conditions: Aeration with 30 - 100 mL/min
- Measuring equipment: Visual check of aeration twice per day
- Details of trap for CO2 and volatile organics if used: CO2 adsorption vessels were connected to the air outlets of the incubation vessels via a series of 3 gas wash bottles, each containing 100 mL of a 0.0125 mol/L Ba(OH)2 solution.
- Other: Application: The necessary amounts of bidistilled water, mineral salts medium and inoculum were placed in each of the incubation vessels. The vessels were aerated for 24 h with CO2 free air. After 24 h the CO2 adsorption vessels were connected to the air outlets of the incubation vessels. The test item and reference item were weighed out. A defined volume of bidistilled water was added to the test item and it was treated with ultrasound. The dispersions and the reference item were transferred into the incubation vessels. The vessels were made up to 3 L with bidistilled water and connected to the system for the production of CO2 free air.
SAMPLING
- Sampling frequency: Backtitration of the residual Ba(OH)2 with 0.05 N HCL was carried out three times a week during the first ten days and thereafter twice weekly.
- Sampling method: For each titration the first gas wash bottle was removed and a new bottle was connected to the last one.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Test medium without test and reference item
- Abiotic sterile control: No
- Toxicity control: Test item and reference item in test concentration
STATISTICAL METHODS:
The theoretical production of carbon dioxide (ThCO2) of the test item and functional control is calculated by the carbon content and the molecular formula, respectively.
The produced CO2 was calculated by: 1 mL HCl (c = 0.05 mol/L) = 1.1 mg CO2
The net amount of CO2 produced is calculated by correcting the results of the test item and functional control for endogenous CO2 production of the inoculum controls.
The biodegradation is calculated from the ratio theoretical CO2 production to net CO2 production.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg/L
Results and discussion
- Preliminary study:
- Not performed
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 4
- Sampling time:
- 28 d
- Details on results:
- The total amount of CO2 produced in 28 days was analysed by titration in 12 measurements.
The adaptation phase of the functional control changed after 3 days into the degradation phase (degradation 10 %). The course of the degradation was rapid and the functional control reached the pass level of 60 % after 9 days and came to a maximum of 95 % after 28 days. The validity criterion degradation 60 % after 14 d is fulfilled.
BOD5 / COD results
- Results with reference substance:
- The adaptation phase of the functional control changed after 2 days into the degradation phase (degradation >/= 10 %). The course of the degradation was rapid and the functional control reached the pass level of 60 % after 6 days and came to 82 % after 14 days. The validity criterion degradation >/= 60 % after 14 d is fulfilled.
Both test item replicates did not reach the 10 % level (beginning of biodegradation) within 28 days. The mean biodegradation rate after 28 days was 4 %.
The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days.
In the toxicity control a biodegradation rate of 73 % was determined within 14 days and it came to 79 % after 28 days. The biodegradation of the reference item was not inhibited by the test item.
Any other information on results incl. tables
CO2-Production and Biodegradation after 28 Days
CO2-Production |
Functional |
Test Item |
Toxicity Control |
|
after 28 d |
Control |
20 mg/L |
20 mg/L Test Item + |
|
20 mg/L |
No.1 |
No. 2 |
20 mg/L Reference Item |
|
Net [mg/3 L] |
121.1 |
2.2 |
5.7 |
271.1 |
[mg/L] |
40.4 |
0.7 |
1.9 |
90.4 |
Theor. [mg/3 L] |
127.8 |
118.2 |
118.2 |
246.0 |
[mg/L] |
42.6 |
39.4 |
39.4 |
82.0 |
Degradation [%] |
95 |
2 |
5 |
100 |
CO2-Production and
Biodegradation in the Inoculum Control, Functional Control
and Toxicity Control Samples
Study Day |
Date |
Inoculum |
Functional Control 20 mg/L |
Toxicity Control 20 mg/L Test Item + |
|
||||
[mg CO2/3L] |
[mg CO2/3L] |
Degr. |
[mg CO2/3L] |
Degr. |
|
||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
|
||
1 |
31.07.09 |
6.0 |
8.9 |
2.9 |
2 |
14.7 |
8.7 |
4 |
|
4 |
03.08.09 |
13.8 |
40.5 |
29.6 |
23 |
54.3 |
49.2 |
20 |
|
6 |
05.08.09 |
12.6 |
34.3 |
51.3 |
40 |
46.9 |
83.5 |
34 |
|
8 |
07.08.09 |
13.2 |
37.1 |
75.2 |
59 |
30.9 |
101.2 |
41 |
|
11 |
10.08.09 |
13.2 |
29.7 |
91.7 |
72 |
54.1 |
142.1 |
58 |
|
14 |
13.08.09 |
18.0 |
26.8 |
100.5 |
79 |
52.8 |
176.9 |
72 |
|
18 |
17.08.09 |
21.6 |
29.9 |
108.8 |
85 |
54.2 |
209.5 |
85 |
|
21 |
20.08.09 |
19.6 |
23.3 |
112.5 |
88 |
51.5 |
241.4 |
98 |
|
25 |
24.08.09 |
20.4 |
26.7 |
118.8 |
93 |
35.3 |
256.3 |
100 |
|
28 |
27.08.09 |
19.1 |
18.6 |
118.8 |
93 |
25.5 |
262.7 |
100 |
|
29* |
28.08.09 |
14.4 |
16.1 |
121.1 |
95 |
22.8 |
271.1 |
100 |
CO2-Production and Biodegradation in the Inoculum Control and Test Item Samples
Study Day |
Date |
Inoculum |
Test Item 20 mg/L |
|||||
Control |
Replicate 1 |
Replicate 2 |
||||||
[mg CO2/3L] |
[mg CO2/3L] |
Degr. |
[mg CO2/3L] |
Degr. |
||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
||
1 |
31.07.09 |
6.0 |
5.4 |
0.0 |
0 |
5.5 |
0.0 |
0 |
4 |
03.08.09 |
13.8 |
16.0 |
2.2 |
2 |
18.9 |
5.1 |
4 |
6 |
05.08.09 |
12.6 |
9.8 |
2.2 |
2 |
6.9 |
5.1 |
4 |
8 |
07.08.09 |
13.2 |
10.8 |
2.2 |
2 |
11.0 |
5.1 |
4 |
11 |
10.08.09 |
13.2 |
12.0 |
2.2 |
2 |
13.8 |
5.7 |
5 |
14 |
13.08.09 |
18.0 |
15.1 |
2.2 |
2 |
14.9 |
5.7 |
5 |
18 |
17.08.09 |
21.6 |
16.1 |
2.2 |
2 |
19.0 |
5.7 |
5 |
21 |
20.08.09 |
19.6 |
17.1 |
2.2 |
2 |
17.1 |
5.7 |
5 |
25 |
24.08.09 |
20.4 |
15.3 |
2.2 |
2 |
18.6 |
5.7 |
5 |
28 |
27.08.09 |
19.1 |
18.6 |
2.2 |
2 |
16.5 |
5.7 |
5 |
29* |
28.08.09 |
14.4 |
13.4 |
2.2 |
2 |
12.1 |
5.7 |
5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days.
- Executive summary:
The ready biodegradability of the test item Chinonbasesulfanilid, trocken was determined with a non adapted activated sludge over a test period of 28 days in the Modified Sturm Test. The study was conducted according to OECD 301 B. The test item was tested at a concentration of 20 mg/L in duplicates, corresponding to a carbon content (TOC) of 10.6 mgC/L in the test vessels. The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29, after residual CO2 had been purged from the test solutions over a period of 24 h. The percentage CO2production was calculated in relation to the theoretical CO2production (ThCO2) of the test item. The biodegradation rate was calculated for each titration time.
To check the activity of the test system, sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % after 9 days and came to a maximum of 95 % after 28 days.
In the toxicity control containing both test and reference item a biodegradation rate of 72 % was determined within 14 days and it came to 100 % after 28 days.The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
The biodegradation of the test item is shown graphically in Figure 1 in comparison to the readily degradable functional control and the toxicity control. Both test item replicates did not reach the 10 % level (beginning of biodegradation) within 28 days. The mean biodegradation rate after 28 days was 4 %.
The test item must be regarded as
not readily biodegradable
in the 10-d-window and after 28 days.Biodegradation of the Test Item Chinonbasesulfanilid, trocken
in Comparison to the Functional Control and Toxicity ControlBiodegradation [%]
Study Day [d]
6
14
21
28
Test Item, 1st Replicate
20 mg/L2
2
2
2
Test Item, 2nd Replicate
20 mg/L4
5
5
5
Functional Control
20 mg/L40
79
88
95
Toxicity Control
20 mg/L Test Item +
20 mg/L Reference Item34
72
98
100
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.