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EC number: 232-808-6 | CAS number: 9026-00-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Partition coefficient
Administrative data
- Endpoint:
- partition coefficient
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 August 2013 - 19 Dec 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certificate included in report
- Type of method:
- shake-flask method to: flask method
- Partition coefficient type:
- octanol-water
Test material
- Reference substance name:
- Lipase, triacylglycerol
- EC Number:
- 232-619-9
- EC Name:
- Lipase, triacylglycerol
- Cas Number:
- 9001-62-1
- Molecular formula:
- Not available. See Remarks.
- IUPAC Name:
- Triacylglycerol lipase IUBMB 3.1.1.3
- Test material form:
- liquid
- Details on test material:
- - Substance type: UVCB
- Physical state: Liquid
- Lot/batch No.: 3606K16
- Expiration date of the lot/batch: Sep 2015
- Storage condition of test material: Minus 20 degrees of C
Constituent 1
Study design
- Analytical method:
- other: UV/VIS spectroscopy
Results and discussion
Partition coefficient
- Key result
- Type:
- log Pow
- Partition coefficient:
- -1.93
- Temp.:
- 20 °C
- pH:
- >= 6.5 - <= 7
- Details on results:
- The detector calibration was found to be linear over the range 0 to 1440 mg/L of standard solutions in water with a regression coefficient of 1.0000.
It had been anticipated that the test substance would not partition into the n-octanol phase and therefore the full scale spectra of the organic phases were assessed in order to verify that the absorbance was due to the enzyme. A typical spectrum is presented in the report and confirms the identity of the material in the n-octanol as lipase.
It was proposed that a reason for the presence of lipase in the n-octanol phases was due to water dissolved in the n-octanol causing the compound to occur in the organic phase. It was therefore regarded that the determined partition coefficient value represented a maximum value and the true partition coefficient was probably lower.
The accuracy of the analytical method was verified by the analysis of five replicate samples prepared at two concentrations: one at the level analysed for the aqueous phases (approximately 1200 mg/L) and one at a level equivalent to a nominal limit of quantification (approximately 21 mg/L). The recoveries were 102% and 85%, respectively, and served to demonstrate the accuracy of the method.
Applicant's summary and conclusion
- Conclusions:
- The octanol/water partition coefficient of lipase was found to be < 0.01 (log10 Pow = < -1.9) at 20°C.
- Executive summary:
A study was performed to determine the partition coefficient of the test substance, lipase, according to EEC Method A8 and OECD Method 107.
The study was conducted to comply with the requirements of European Council Directive for 91/414/EEC (Annex II) as amended by European Commission Directive 94/37/EC and amended by Commission Regulation (EU) No. 544/2011 Implementing Regulation (EC) Nov 1107/2009 of the European Parliament and of the Council as regards the data requirements for active substances.
The octanol/water partition coefficient of lipase was found to be < 0.01 (log10 Pow = < -1.9).
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