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EC number: 605-140-1 | CAS number: 158237-07-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 - 06 Jan 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EC L 133, Part C: Biodegradability: Test for inhibition of oxygen consumption
- Version / remarks:
- corresponds for the most part to the test method OECD 209
- Principles of method if other than guideline:
- A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was directly weighed in. The test substance has been added to about 130 mL deionised water and stirred over night before testing (equilibration phase). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
aeration tank of a waste water plant treating predominantly domestic sewage (Wupper area water authority, Germany)
- Pretreatment: none
- Initial biomass concentration: 480 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 18 - 22 °C
- pH:
- 7.7 (pH of the suspension before application)
- Nominal and measured concentrations:
- control, 100, 1000, and 10000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Aeration: yes, permanent
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionsed water - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- EC50 (30 min) = 10mg/L
- Reported statistics and error estimates:
- The EC values are determined by statistical methods (probit analysis).
- Validity criteria fulfilled:
- not applicable
Reference
Table 1: Measured values of test substance / control
|
Test concentration [mg/L] |
Initial concentration [mg/250 ml] |
O2-start [mg O2/L] |
O2-end [mg O2/L] |
Time [min.] |
Temp. [°C] |
pH |
Test substance |
100 |
25 |
5.2 |
2.7 |
5 |
18.9 |
7.7 |
1000 |
250 |
5.3 |
2.5 |
6 |
19.5 |
7.9 |
|
10000 |
2500 |
4.7 |
2.9 |
4 |
19.1 |
7.8 |
|
Control 1 |
|
|
4.9 |
2.9 |
4 |
18.9 |
7.7 |
Control 2 |
|
|
5.4 |
2.8 |
5 |
19.1 |
7.8 |
Physiochemical oxygen consumption control |
10000 |
2500 |
8.0 |
8.0 |
6 |
19.7 |
7.6 |
Because of strong respiration of the activated sludge only 0.48 g/L SS were used.
The test substance has been added to about 130 mL deionised water and stirred over night before testing (equilibration phase).
Table 2: Measured values of reference substance
|
Test concentration [mg/L] |
Initial Concentration* [mg/250 ml] |
O2-start [mg O2/L] |
O2-end [mg O2/L] |
Time [min.] |
Temp. [°C] |
pH |
Reference substance |
5 |
2.5 |
6.2 |
3.9 |
6 |
18.8 |
7.7 |
10 |
5 |
6.2 |
4.9 |
5 |
18.7 |
7.7 |
|
20 |
10 |
7.8 |
7.0 |
6 |
18.9 |
7.8 |
* Volume added in ml of 3.5-Dichlorophenol stock solution (500 mg/L); total volume 250 mL.
Table 3: Results with test substance
Test concentration [mg/L] |
Respiratory rate test substance[mg/L x h] |
Physiochemical oxygen consumption [mg/L x h] |
Respiratory rate -Physiochemical oxygen consumption [mg/L x h] |
Inhibition [%] |
100 |
30.0 |
0.0* |
30.0 |
2.0 |
1000 |
28.0 |
0.0* |
28.0 |
8.5 |
10000 |
27.0 |
0.0 |
27.0 |
11.8 |
Control mean |
30.6 |
|
||
Control 1 |
30.0 |
|||
Control 2 |
31.2 |
*The physico chemical oxygen consumption is determined at 10000 mg/L test substance. No physico-chemical oxygen consumption has been determined. Therefore lower concentrations of the test substance cause no physico-chemical oxygen consumption (deduced values).
Table 4: Results with reference substance
Test concentration [mg/L] |
Respiratory rate [mg/L x h] |
Inhibition [%] |
5 |
23.0 |
24.8 |
10 |
15.6 |
49.0 |
20 |
8.0 |
73.9 |
The reference substance concentration result from the quantities used in the preparations and have not been confirmed by analytical methods.
Description of key information
NOEC (30 min) ≥ 10000 mg/L (nominal, similar to OECD 209, activated sludge)
Key value for chemical safety assessment
Additional information
One experimental study is available testing the toxicity of the substance towards aquatic microorganisms (1998). The study was conducted with activated sludge. The study procedure followed the 'Commission Directive 88/302/EEC, Official Journal of the EC L 133, Part C: Biodegradability: Test for inhibition of oxygen consumption' which corresponds for the most part to the test method OECD 209. A defined quantity of activated sludge was mixed with synthetic medium and the respiratory rate was measured over time. This rate is compared to those measured in test preparations with various concentrations of the test substance. Activated sludge was exposed to nominal test item concentrations of 100, 1000, and 10000 mg/L for 30 min. Since no respiratory inhibition was observed compared to the control a NOEC (30 min) ≥10000 mg/L was derived.
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