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EC number: 689-188-9 | CAS number: 149343-84-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7 April 1993 to 17 June 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- OECD Guidelines for the Testing of Chemicals, Protocol No. 471
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- Method B14 in Commission Directive 92/69/EEC
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Cuprate(4-), [C-(aminosulfonyl)-C-[[[2-[[4-chloro-6-[(2,5-disulfophenyl)amino]-1,3,5-triazin-2-yl]amino]ethyl]amino]sulfonyl]-29H,31H-phthalocyanine-C,C-disulfonato(6-)-N29,N30,N31,N32]-, sodium salts
- EC Number:
- 689-188-9
- Cas Number:
- 149343-84-0
- Molecular formula:
- C32H24N12O8S4Cu to C76H56N28O32S12Cl4Na8Cu
- IUPAC Name:
- Cuprate(4-), [C-(aminosulfonyl)-C-[[[2-[[4-chloro-6-[(2,5-disulfophenyl)amino]-1,3,5-triazin-2-yl]amino]ethyl]amino]sulfonyl]-29H,31H-phthalocyanine-C,C-disulfonato(6-)-N29,N30,N31,N32]-, sodium salts
- Test material form:
- solid: particulate/powder
- Details on test material:
- Identification: JPR Blue 100
Description: Blue powder
Chemical Name: Cuprate(2-),[2-[[4-chloro-6-[[2-[(29H,31H-phthalocyaninylsulphonyl)amino]ethyl]amino]-1,3,5-triazin-2-yl]amino]-1,4-benzenedisulphonato(4-)-N29,N30,N31,N32]-mono(or bis)aminosulphonyl mono (or di)sulpho derivs., sodium salts.
Lot Number: 303001
Purity: 95.2%
Major Impurities: H2o, NaCl, Na2SO4
Date Received: 30 March 1993
Container: Opaque plastic jar x 7
Storage conditions: Room temperature
Constituent 1
- Specific details on test material used for the study:
- No further details specified in the study report.
Method
- Target gene:
- histidine and tryptophan
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Preliminary Toxicity Study: 0, 312.5, 625, 1250, 2500, 5000 μg/plate
Experiment 1: 0, 8.0, 40, 200, 1000, 5000 μg/plate
Experiment 2: 0, 312.5, 625, 1250, 2500, 5000 μg/plate
Appropriate dose levels used in the main study set following the preliminary toxicity study and in accordance with the test guidelines. - Vehicle / solvent:
- JPR Blue 100 was accurately weighed (with an allowance made for purity) and dissolved in sterile distilled water (lot number 301005 108 Exp. 2/96) and appropriate dilutions made.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- N-ethyl-N-nitro-N-nitrosoguanidine
- benzo(a)pyrene
- other: 2-Aminoanthracene (2AA)
- Details on test system and experimental conditions:
- Microsomal Enzyme Fraction
Lot No. Aro. S9/10/05/93, prepared on 10/05/93 and Aro. S9/23/04/ 93, prepared on 23/04/93, were obtained from the British Industrial Biological Research Association on 11/ 05/93. They were prepared from the livers of male Sprague-Dawley rats weighing ~ 200 g. These had received a single i.p. injection of Aroclor 1254 at 500 mg/kg, 5 days before S9 preparation.
The S9 was stored at -196 °C in a Statebourne liquid nitrogen freezer, model SXR 34.
S9 Mix and Agar
The S9 Mix was prepared at 4 °C as follows:
S9: 5.0 ml
1.65 M KCl / 0.4 M MgCl2: 1.0 ml
0.1 M Glucose-6-phosphate: 2.5 ml
0.1 M NADP: 2.0 ml
0.2 M Sodium phosphate buffer (pH 7.4): 25.0 ml
Sterile distilled water: 14.5 ml
Top agar was prepared using 0.6% Difco Bacto agar (lot number 801679 10/ 96) and 0.5% sodium chloride. For the Salmonella strains 5 ml of 1.0 mM histidine/1.0 mM biotin solution was added to each 100 ml of top agar and for the Escherichia coli strain 5 ml of 1.0 mM tryptophan was added to each 100 ml of top agar. Base agar plates were prepared using 1.2% Oxoid Agar Technical No.3 (lot number 225 62262 7/97 experiment 1 and lot number 336 66640 10/97 experiment 2) with Vogel-Bonner Medium E and 20 mg/ ml 0-glucose.
Test Procedure
a) Preliminary Toxicity Study
In order to select appropriate dose levels for use in the main study, a preliminary test was carried out to determine the toxicity of the test material to the tester organisms. 0.1 ml of bacterial suspension (TA100 or WP2uvrA-), 2 ml of molten, trace histidine or tryptophan supplemented media (histidine/ biotin or tryptophan and top agar), 0.1 ml of test solution and 0.5 ml of phosphate buffer were overlayed onto sterile plates of Vogel -Bonner minimal agar (30 ml / plate). Five doses of the test material and a solvent control (sterile distilled water) were tested in duplicate. After 48 hours incubation at 37 °C the plates were scored for revertant colonies and examined for a thinning of the background lawn.
b) Mutation Study
EXPERIMENT 1
Five concentrations of the test material were assayed in triplicate against each tester strain using the direct plate incorporation method in accordance with the standard methods for mutagenicity tests using bacteria.
Test Material and Negative Controls
0.1 ml aliquots of one of the bacterial suspensions were dispensed into sets of sterile test tubes followed by 2.0 ml of molten trace histidine (or tryptophan in the case of WP2uvrA-) supplemented top agar at 45 °C. These sets comprised two test tubes for each bacterial tester strain. 0.1 ml of the appropriately diluted test material or negative control solution was also added to each of the two tubes, followed by either 0.5 ml of the S9 liver microsome mix or 0.5 ml of pH 7.4 buffer. The contents of each test tube were mixed and equally distributed onto the surface of Vogel-Bonner agar plates (one tube per plate). This procedure was repeated, in triplicate, for each bacterial strain and for each concentration of test material.
Positive Controls
i) Without Activation
0.1 ml of one of the positive control solutions (ENNG, 9AA or 4NQO) was added to a test tube containing 2.0 ml of molten, trace histidine or tryptophan supplemented top agar and 0.1 ml of the appropriate bacterial suspension. Finally, 0.5 ml of pH 7.4 buffer was added to the tube, the contents mixed and poured onto agar plates. This procedure was then repeated in triplicate, for each of the positive controls.
ii) With Activation
0.1 ml of 2AA or BP solution was added to a test tube containing 2.0 ml of molten, trace histidine or tryptophan supplemented top agar and 0.1 ml of one of the test bacterial suspensions. Finally, 0.5 ml of S9 mix was added to the test tube, the contents mixed and poured onto an agar plate. This procedure was then repeated, in triplicate, for each tester strain.
The plates were incubated at 37 °C for 48 hours and the number of revertant colonies counted.
EXPERIMENT 2
The second experiment was performed using fresh bacterial cultures, test material and control solutions in triplicate. - Rationale for test conditions:
- In accordance with test guidelines
- Evaluation criteria:
- For a substance to be considered positive in this test system, it should have induced a dose-related and significant increase in mutation rate (of at least twice the spontaneous reversion rate) in one or more strains of bacteria in the presence and/or absence of the S9 microsomal enzymes in both experiments at sub-toxic dose levels. If the two experiments give conflicting results then a third experiment may be used to confirm the correct response. To be considered negative the number of induced revertants compared to spontaneous revertants should be less than twofold at each dose level employed, the intervals of which should be between 2 and 5 fold and extend to the limits imposed by toxicity or solubility or up to the maximum recommended dose of 5000 μg/plate.
In this case the limiting factor was the maximum recommended dose. - Statistics:
- Not specified
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- Preliminary Toxicity Study
The dose range of JPR Blue 100 used in the preliminary toxicity study was 0, 312 .5, 625, 1250, 2500 and 5000 μg/plate. JPR Blue 100 was non-toxic in the strains of bacteria used (TA100 and WP2uvrA-).
Mutation Study
The results of the checks for characteristics, viability and spontaneous reversion rate for each tester strain were all found to be satisfactory.
No toxicity was exhibited to any of the strains of bacteria used.
No significant increases in the numbers of revertant colonies of bacteria were recorded for any of the strains of bacteria used, at any dose level, either with or without metabolic activation.
The positive control substances all produced marked increases in the number of revertant colonies and the activity of the S9 fraction was found to be satisfactory.
Any other information on results incl. tables
Preliminary Toxicity Study
The mean number of revertant colonies for the toxicity assay were:
Strain |
Dose (μg/plate) |
|||||
0 |
312.5 |
625 |
1250 |
2500 |
5000 |
|
TA100 WP2uvrA- |
158.5 38.5 |
176.5 38.0 |
170.5 35.5 |
175.5 36.0 |
172.5 36.0 |
163.0 33.5 |
KEY TO TABLES OF TEST RESULTS
NOTES:
1. When bacterial growth inhibition is found, the applicable value is marked with an asterisk.
2. The average number of colonies for each concentration is recorded in parenthesis.
3. “Number of revertants” – The observed values and average value are shown in order, beginning with the low concentration of the test substance.
4. The following postfixes are used when required:-
C = contaminated
P = precipitate
X = plate unscorable
ENNG = N-ethyl-N’-nitro-N-nitrosoguanidine
4NQO = 4-Nitroquinoline-1-oxide
9AA = 9-Aminoacridine
2AA = 2-Aminoanthracene
BP = Benzo(a)pyrene
TABLE OF TEST RESULTS: EXPERIMENT 1
NAME OF TEST SUBSTANCE: JPR BLUE 100
WITH (+) OR WITHOUT (-) S9 MIX |
TEST SUBSTANCE CONCENTRATION (μg/plate) |
NUMBER OF REVERTANTS (number of colonies/plate) |
|||||||||
BASE-PAIR SUBSTITUTION TYPE |
FRAMESHIFT TYPE |
||||||||||
TA100 |
TA1535 |
WP2uvrA- |
TA98 |
TA1537 |
|||||||
- |
0 |
144 144 166 |
(151.3) |
14 9 13 |
(12.0) |
10 14 9 |
(11.0) |
32 27 24 |
(27.7) |
5 12 9 |
(8.7) |
- |
8.0 |
143 167 167 |
(159.0) |
10 11 9 |
(10.0) |
9 14 13 |
(12.0) |
16 22 26 |
(21.3) |
7 7 6 |
(6.7) |
- |
40 |
145 120 152 |
(139.0) |
10 10 10 |
(10.0) |
11 9 13 |
(11.0) |
26 22 22 |
(23.3) |
7 5 6 |
(6.0) |
- |
200 |
135 166 144 |
(148.3) |
11 14 14 |
(13.0) |
9 12 15 |
(12.0) |
21 24 24 |
(23.0) |
6 6 9 |
(7.0) |
- |
1000 |
145 166 161 |
(157.3) |
13 10 9 |
(10.7) |
13 13 18 |
(14.7) |
28 22 19 |
(23.0) |
8 7 7 |
(7.3) |
- |
5000 |
141 160 163 |
(154.7) |
10 13 10 |
(11.0) |
16 14 13 |
(14.3) |
22 26 20 |
(22.7) |
9 8 5 |
(7.3) |
POSITIVE CONTROL NOT REQUIRING S9 MIX |
NAME |
ENNG |
ENNG |
ENNG |
4NQO |
9AA |
|||||
CONCENTRATION (μg/plate) |
3.0 |
5.0 |
2.0 |
0.2 |
80.0 |
||||||
- |
NUMBER OF COLONIES/PLATE |
508 531 533 |
(557.3) |
149 152 139 |
(146.7) |
276 254 266 |
(265.3) |
134 129 170 |
(144.3) |
1072 1003 1064 |
(1046.3) |
TABLE OF TEST RESULTS: EXPERIMENT 1 (contd)
NAME OF TEST SUBSTANCE: JPR BLUE 100
WITH (+) OR WITHOUT (-) S9 MIX |
TEST SUBSTANCE CONCENTRATION (μg/plate) |
NUMBER OF REVERTANTS (number of colonies/plate) |
|||||||||
BASE-PAIR SUBSTITUTION TYPE |
FRAMESHIFT TYPE |
||||||||||
TA100 |
TA1535 |
WP2uvrA- |
TA98 |
TA1537 |
|||||||
+ |
0 |
156 158 176 |
(163.3) |
11 11 17 |
(13.0) |
11 19 9 |
(13.0) |
27 23 18 |
(22.7) |
9 9 7 |
(8.3) |
+ |
8.0 |
149 162 167 |
(159.3) |
11 16 11 |
(12.7) |
20 15 13 |
(16.0) |
19 22 23 |
(21.3) |
14 9 14 |
(12.3) |
+ |
40 |
145 141 140 |
(142.0) |
15 15 10 |
(13.3) |
16 13 10 |
(13.0) |
25 23 20 |
(22.7) |
6 10 7 |
(7.7) |
+ |
200 |
136 162 133 |
(143.7) |
10 10 16 |
(12.0) |
13 14 13 |
(13.3) |
29 24 24 |
(25.7) |
13 5 10 |
(9.3) |
+ |
1000 |
148 158 161 |
(155.7) |
11 14 14 |
(13.0) |
15 19 16 |
(16.7) |
19 19 25 |
(21.0) |
6 10 9 |
(8.3) |
+ |
5000 |
149 155 171 |
(158.3) |
13 9 13 |
(11.7) |
13 12 17 |
(14.0) |
20 23 23 |
(22.0) |
8 10 8 |
(8.7) |
POSITIVE CONTROL REQUIRING S9 MIX |
NAME |
BP |
2AA |
2AA |
BP |
BP |
|||||
CONCENTRATION (μg/plate) |
5.0 |
2.0 |
10.0 |
5.0 |
5.0 |
||||||
+ |
NUMBER OF COLONIES/PLATE |
401 446 420 |
(422.3) |
183 132 172 |
(162.3) |
210 211 204 |
(208.3) |
181 157 156 |
(164.7) |
99 107 110 |
(105.3) |
TABLE OF TEST RESULT: EXPERIMENT 2
NAME OF TEST SUBSTANCE: JPR BLUE 100
WITH (+) OR WITHOUT (-) S9 MIX |
TEST SUBSTANCE CONCENTRATION (μg/plate) |
NUMBER OF REVERTANTS (number of colonies/plate) |
|||||||||
BASE-PAIR SUBSTITUTION TYPE |
FRAMESHIFT TYPE |
||||||||||
TA100 |
TA1535 |
WP2uvrA- |
TA98 |
TA1537 |
|||||||
- |
0 |
179 146 184 |
(169.7) |
10 16 11 |
(12.3) |
26 16 15 |
(19.0) |
20 16 15 |
(17.0) |
9 9 10 |
(9.3) |
- |
312.5 |
175 150 160 |
(161.7) |
15 11 11 |
(12.3) |
15 13 11 |
(13.0) |
15 16 21 |
(17.3) |
9 10 11 |
(10.0) |
- |
625 |
150 170 162 |
(160.7) |
10 12 13 |
(11.7) |
19 18 20 |
(19.0) |
16 15 18 |
(16.3) |
8 9 14 |
(10.3) |
- |
1250 |
145 146 166 |
(152.3) |
11 9 15 |
(11.7) |
19 14 11 |
(14.7) |
17 15 22 |
(18.0) |
9 11 9 |
(9.7) |
- |
2500 |
150 146 171 |
(155.7) |
16 12 16 |
(14.7) |
17 17 15 |
(16.3) |
16 18 21 |
(18.3) |
9 12 11 |
(10.7) |
- |
5000 |
151 169 144 |
(154.7) |
11 14 14 |
(13.0) |
9 15 18 |
(14.0) |
19 14 19 |
(17.3) |
10 7 12 |
(9.7) |
POSITIVE CONTROL NOT REQUIRING S9 MIX |
NAME |
ENNG |
ENNG |
ENNG |
4NQO |
9AA |
|||||
CONCENTRATION (μg/plate) |
3.0 |
5.0 |
2.0 |
0.2 |
80.0 |
||||||
- |
NUMBER OF COLONIES/PLATE |
751 707 709 |
(722.3) |
179 161 136 |
(158.7) |
421 427 462 |
(436.7) |
154 174 153 |
(160.3) |
691 701 755 |
(715.7) |
TABLE OF TEST RESULTS: EXPERIMENT 2 (contd)
NAME OF TEST SUBSTANCE: JPR BLUE 100
WITH (+) OR WITHOUT (-) S9 MIX |
TEST SUBSTANCE CONCENTRATION (μg/plate) |
NUMBER OF REVERTANTS (number of colonies/plate) |
|||||||||
BASE-PAIR SUBSTITUTION TYPE |
FRAMESHIFT TYPE |
||||||||||
TA100 |
TA1535 |
WP2uvrA- |
TA98 |
TA1537 |
|||||||
+ |
0 |
184 174 124 |
(160.7) |
15 13 14 |
(14.0) |
20 16 11 |
(15.7) |
26 23 23 |
(24.0) |
13 14 11 |
(12.7) |
+ |
312.5 |
144 132 165 |
(147.0) |
9 15 15 |
(13.0) |
C 18 13 |
(15.5) |
23 20 19 |
(20.7) |
13 11 8 |
(10.7) |
+ |
625 |
148 137 148 |
(144.3) |
14 11 18 |
(14.3) |
18 19 13 |
(16.7) |
25 23 22 |
(23.3) |
14 7 7 |
(9.3) |
+ |
1250 |
159 149 144 |
(150.7) |
18 19 14 |
(17.0) |
10 16 14 |
(13.3) |
16 17 23 |
(18.7) |
7 8 7 |
(7.3) |
+ |
2500 |
145 161 158 |
(154.7) |
11 15 16 |
(14.0) |
18 18 19 |
(18.3) |
19 24 25 |
(22.7) |
9 11 15 |
(11.7) |
+ |
5000 |
170 155 161 |
(162.0) |
10 14 15 |
(13.0) |
16 14 18 |
(16.0) |
22 24 19 |
(21.7) |
7 8 12 |
(9.0) |
POSITIVE CONTROL REQUIRING S9 MIX |
NAME |
BP |
2AA |
2AA |
BP |
BP |
|||||
CONCENTRATION (μg/plate) |
5.0 |
2.0 |
10.0 |
5.0 |
5.0 |
||||||
+ |
NUMBER OF COLONIES/PLATE |
457 458 395 |
(436.7) |
161 178 161 |
(166.7) |
137 141 133 |
(137.0) |
161 178 187 |
(175.3) |
98 119 120 |
(112.3) |
Applicant's summary and conclusion
- Conclusions:
- The test material, JPR Blue 100, was found to be non-mutagenic under the conditions of this test.
- Executive summary:
Salmonella typhimurium strains TA1535, TA1537, TA98 and TA100 and Escherichia coli strain WP2uvrA- were treated with JPR Blue 100 by the Ames plate incorporation method at five dose levels, in triplicate both with and without the addition of a rat liver homogenate metabolizing system at 10% in standard co-factors. This method conforms to the guidelines for bacterial mutagenicity testing published by the major Japanese Regulatory Authorities including MITI, MHW, MOL and MAFF. It also meets the requirements of the OECD, EEC and USA, EPA (TSCA) guidelines. The dose range was determined in a preliminary toxicity assay and was 8 to 5000μg/platein the first experiment. The experiment was repeated on a separate day using different cultures of the bacterial strains and fresh chemical formulations. In this case the dose range of JPR Blue 100 was 312.5 to 5000μg/plate.
The solvent (sterile distilled water) control plates gave counts of revertant colonies within the normal range.
All positive control chemicals produced marked increases in the numbers of revertant colonies, both with and without the metabolising system.
JPR Blue 100 caused no reduction in the growth of the bacterial lawn at any dose level either with or without metabolic activation. JPR Blue 100 was therefore tested up to the maximum recommended dose level of 5000μg/ plate.
No significant increase in the numbers of revertant colonies was recorded for any of the bacterial strains with any dose of JPR Blue 100, either with or without metabolic activation. JPR Blue 100 was found to be non-mutagenic under the conditions of this test.
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.