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EC number: 689-188-9 | CAS number: 149343-84-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 - 26 May 1993 .
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Guidelines for Testing of Chemicals (1984) No . 202 "Daphnia sp. Acute Immobilisation Test and Reproduction Test"
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Method C.2 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/548/EEC).
- Deviations:
- not specified
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- No further details specified in the study report.
- Analytical monitoring:
- yes
- Details on sampling:
- Verified by chemical analysis of the control and the 100 mg/l test groups at 0 and 48 hours
- Vehicle:
- no
- Details on test solutions:
- Direct dispersion in water. 200 mg of test material was dispersed in aged and dechlorinated tap water with the aid of ultrasonic disruption and the volume adjusted to 2 litres to give a 100 mg/l test concentration.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Name: Daphnia magna Straus.
Source: Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquee, (I.R.CH.A}, France.
Culture: At 21 °C in polypropylene vessels containing two litres of aged and dechlorinated tap water. Cultures were fed daily with a suspension of mixed algae (predominantly Chlorella spp.}. Culture conditions ensure that reproduction is by parthenogenesis.
Selection: Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation period specified in the study report.
- Hardness:
- approximately 50 mg/l as Caco3.
- Test temperature:
- 21 °C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- 7.9 -8.2 mg O2/l
- Salinity:
- Not applicable-freshwater study
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Verification of test concentrations showed the measured concentrations from analysis at 0 and 48 hours to be near nominal. It was therefore considered justifiable to estimate the Ec 50 values based on nominal test concentrations only .
- Details on test conditions:
- Test vessels: Glass jars each containing 200 ml test solution and covered to reduce evaporation.
Experimental design: 1 test concentration of 4 replicates plus 1 control in duplicate. 10 animals per concentration.
This study design conforms to a Limit Test to show no effect on the test organisms at the highest recommended test concentration in the OECD/ EEC test guidelines.
Method of initiation: Daphnia were placed in the test solutions after addition of the test material.
Loading: 20 ml test solution per organism.
Photoperiod: 16 h light : 8 h dark.
Temperature: 21.0 °C
Aeration: None.
Test concentration: 100 mg/l, 4 replicates. This test concentration was assigned following a preliminary range-finding study where 10 daphnids were exposed for a period of 48 hours to each of the following test concentrations: 0.10, 1. 0, 10 and 100 mg/l. The results showed no effect at all the test concentrations thereby indicating the choice of test concentration employed in the test.
Medium renewal: None.
Duration of exposure: 48 hours.
Environmental Measurements: Temperature was recorded daily. pH and dissolved oxygen concentrations were recorded at initiation and termination of the study .
Criteria of effect: Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- There were no immobilised Daphnia or other adverse reactions to exposure in 40 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.
Inspection of the immobility data gave the following results based on nominal test concentrations:
Time (h) EC50 (mg/l)
24 >100
48 >100
"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.
"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.
The “No Observed Effect Concentration" is based upon zero immobilisation at this test concentration.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l. - Results with reference substance (positive control):
- Not specified
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Inspection of the immobility data gave the following results based on nominal test concentrations:
Time (h) EC50 (mg/l)
24 >100
48 >100
"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.
"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l. - Executive summary:
TEST SPECIES: Daphnia magna Straus tested in the 1st instar.
TEST TYPE: Acute toxicity. 48h EC50 (immobilisation).
TEST CONDIT IONS: Static test conditions according to OECD Guideline No. 202, referenced as Method C.2 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC).
RESULTS
There were no immobilised Daphnia or other adverse reactions to exposure in 40 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.
The following results are based on nominal test concentrations:
Time (h) EC50 (mg/l)
24 >100
48 >100
"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.
"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.
Reference
Cumulative Immobilisation Data for Daphnia magna Exposed for 48 Hours to JPR Blue 100
Nominal Concentration (mg/l)
|
Cumulative immobilized Daphnia (initial population: 10 per replicate) |
|||||
24 hours |
48 hours |
|||||
No. per replicate |
Total |
% |
No. per replicate |
Total |
% |
|
Control R1 R2 |
0 0 |
0 |
0 |
0 0 |
0 |
0 |
100 R1 R2 R3 R4 |
0 0 0 0 |
0 |
0 |
0 0 0 0 |
0 |
0 |
R1– R4= Replicates 1 – 4
Environmental Measurements for Daphnia magna Exposed for 48 hours for JPR Blue 100
Nominal Concentration (mg/l) |
0 hours |
24 hours |
48 hours |
||||
pH |
mg O2/l |
T °C |
T °C |
pH |
mg O2/l |
T °C |
|
Control R1 R2 |
7.8 7.8 |
8.2 8.2 |
21.0 21.0 |
21.0 21.0 |
7.7 7.7 |
7.9 7.9 |
21.0 21.0 |
100 R1 R2 R3 R4 |
7.8 7.8 7.8 7.8 |
8.2 8.2 8.2 8.2 |
21.0 21.0 21.0 21.0 |
21.0 21.0 21.0 21.0 |
7.7 7.7 7.7 7.7 |
7.9 7.9 7.9 7.9 |
21.0 21.0 21.0 21.0 |
R1– R4= Replicates 1 - 4
Description of key information
The following results are based on nominal test concentrations:
Time (h) EC50 (mg/l)
24 >100
48 >100
"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
TEST SPECIES: Daphnia magna Straus tested in the 1st instar.
TEST TYPE: Acute toxicity. 48h EC50 (immobilisation).
TEST CONDIT IONS: Static test conditions according to OECD Guideline No. 202, referenced as Method C.2 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC).
RESULTS
There were no immobilised Daphnia or other adverse reactions to exposure in 40 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.
The following results are based on nominal test concentrations:
Time (h) EC50 (mg/l)
24 >100
48 >100
"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.
"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.
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