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EC number: 201-762-9 | CAS number: 87-66-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: method described by Gerberick and Ryan (1989)
- Deviations:
- yes
- Remarks:
- modification of the method was used but it was not detailed
- Principles of method if other than guideline:
- - Principle of test:
t has been noted that some irritants can also induce proliferation in the draining lymph nodes, thereby manifesting as false positives in the LLNA (Montelius et al., 1994; Basketter et al., 1998). Thus, it was important to determine whether irritation cont ributed to the
positive responses observed in the LLNA.
- Short description of test conditions: The Authors tried to establish the minimal irritating concentration (i.e. the lowest concentration of chemical to elicit a significantly greater ear swelling response than that observed in the vehicle control group) and the maximal non-irritating concentration (the
highest concentration producing ear swelling that is not significantly different from the vehicle control).
- Parameters analysed / observed: The percentage of ear swelling was calculated for each mouse as the [(mean thickness of the post-treated
ear/mean thickness of the pre-treated ear) × 100]−100. - GLP compliance:
- not specified
- Remarks:
- refer to section "any other information on materials and methods" for remarks related to GLP compliance for a test performed after 2008, 1st June
Test material
- Reference substance name:
- Pyrogallol
- EC Number:
- 201-762-9
- EC Name:
- Pyrogallol
- Cas Number:
- 87-66-1
- Molecular formula:
- C6H6O3
- IUPAC Name:
- benzene-1,2,3-triol
- Reference substance name:
- unknown impurities
- IUPAC Name:
- unknown impurities
- Test material form:
- not specified
- Details on test material:
- not specified
Constituent 1
impurity 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material:
purchased from Sigma (St. Louis, MO).
- Expiration date of the lot/batch:
not specified
- Purity test date: not specified
RADIOLABELLING INFORMATION (if applicable)
not applicable
- Radiochemical purity:
-
- Specific activity: -
- Locations of the label: -
- Expiration date of radiochemical substance:
-
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
not specified
- Stability under test conditions:
not specified
- Solubility and stability of the test substance in the solvent/vehicle:
Initial studies on the solubility of PYR demonstrated that the compound was soluble at concentrations of 75% (w/v) and below in acetone:olive oil
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
not specified
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
not specified
- Preliminary purification step (if any):
not specified
- Final dilution of a dissolved solid, stock liquid or gel:
Test solutions were prepared daily in the vehicle 4:1 acetone:olive oil (AOO) as recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM; Dean et al., 2001).
- Final preparation of a solid:
not relevant
FORM AS APPLIED IN THE TEST (if different from that of starting material)
liquid
TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)
not applicable
OTHER SPECIFICS: white odorless crystal (FW 126.11, CAS No.87-66-1)
Test animals
- Species:
- mouse
- Strain:
- Balb/c
Test system
- Type of coverage:
- not specified
- Preparation of test site:
- shaved
- Vehicle:
- other: acetone: olive oil
- Controls:
- other: 0.15% 2,4-dinitrofluorobenzene (DNFB) and 25% hexyl cinnamic aldehyde (HCA)
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): Each mouse received 12.5 μl of test chemical on both sides of each ear
- Concentration (if solution): 0,125%, 0,25%, 1%, 5%, 10%
VEHICLE
- Amount(s) applied (volume or weight with unit): Each mouse received 12.5 μl ofvehicle on both sides of each ear
- Concentration (if solution): not specified
- Lot/batch no. (if required): not specified
- Purity: not specified
NEGATIVE CONTROL
- Amount(s) applied (volume or weight): Naïve animals were left untreated
- Concentration (if solution): not specified
POSITIVE CONTROL
- Amount(s) applied (volume or weight): not specified
- Concentration (if solution): not specified - Duration of treatment / exposure:
- four consecutive days.
- Observation period:
- 24 (± 2) hrs
- Number of animals:
- 6–8 mice for each treatment group
Results and discussion
In vivo
Results
- Irritation parameter:
- other: positive irritant response
- Basis:
- mean
- Time point:
- 24 h
- Reversibility:
- not specified
- Remarks on result:
- positive indication of irritation
- Irritant / corrosive response data:
- PYR is irritating to the skin at concentrations as low as 0.125%.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- PYR induced a positive response in the IRR at concentrations as low as 0.125%.
Because PYR also induced a positive irritant response, it should be classified as an irritant
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