3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption, other

Remarks:

Estimation of adsorption using HPLC method

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 Mar 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

25 ± 1 °C

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: HPLC LC-2010CHT (Shimadzu Corporation)
- Type, material and dimension of analytical (guard) column: Inertsil CN-3 (150 mm x 2.1 mm i.d., particle size 5 µm, GL Sciences Inc.)
- Detection system: UV-VIS detector, wavelength: 210 nm for reference item, 200 nm for test item

MOBILE PHASES
- Type: Methanol/purified water (60/40 v/v), flow rate: 0.2 mL/min
- Solutes for dissolving test and reference substances: methanol

DETERMINATION OF DEAD TIME
- Method: Dead time was determined by injection of formamide (CAS 75-12-7, purity ≥ 98.5%)

REFERENCE SUBSTANCES
- Methyl benzoate (CAS 93-58-3, purity ≥ 98.0%)
- Linuron (CAS 330-55-2, purity 99.8%)
- Naphthalene (CAS 91-20-3, purity ≥ 99%)
- Fenthion (CAS 55-38-9, purity 97.9%)
- Phenanthrene (CAS 85-01-8, purity 98%)
- DDT (CAS 50-29-3, purity ≥ 98%)

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 2 µL
- Quantity of reference substances: 2 µL
- Intervals of calibration: The system was calibrated by measuring each reference item in duplicate to prepare a regression line.

REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k': The capacity factor was calculated from the retention time (k = (retention time of reference item - dead time)/dead time).
- Calculation of retention times: The retention times of the reference items and the test item were measured directly.
- Determination of the log Koc value: The log Koc was calculated from the regression equation of the regression line. The average of the two measurements were used as the log Koc value.

Sample No.:

#1

Type:

log Koc

Value:

2.65 dimensionless

Temp.:

25 °C

Remarks on result:

other: Mean of two replicates

Details on results (HPLC method):

- Retention times of reference substances used for calibration: See "Any other information on results incl. tables"
- Details of fitted regression line (log k' vs. log Koc): r²: 0.981, log Koc = 3.416 x log k' + 2.760
- Average retention data for test substance: 4.12 min (first run), 4.10 min (second run)

Table 1: Results from measurements

	Name	Retention time [min]	Capacity factor (k)	Log k	Log Koc
Reference substances	Formamide	2.13	Dead time t0 = 2.13 min
		2.13
	Methyl benzoate	3.06	0.437	-0.360	1.80
		3.06	0.437	-0.360	1.80
	Linuron	3.98	0.869	-0.061	2.59
		3.98	0.869	-0.061	2.59
	Naphthalene	4.82	1.263	0.101	2.75
		4.82	1.263	0.101	2.75
	Fenthion	5.38	1.526	0.184	3.31
		5.38	1.526	0.184	3.31
	Phenanthrene	8.06	2.784	0.445	4.09
		8.07	2.784	0.445	4.09
	DDT	14.03	5.587	0.747	5.63
		14.05	5.596	0.748	5.63
Test item	[trade name]	4.12	0.934	-0.030	2.66
		4.10	0.925	-0.034	2.64

Validity criteria fulfilled:

not applicable

Remarks:

The guideline does not specify validity criteria.

Description of key information

log Koc: 2.65 (OECD 121)

Additional information

One experimental study is available investigating the adsorption potential of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene (CAS 25291-17-2) to soil and sewage sludge (Daikin Industries, 2017). The study was performed according to OECD 121 (GLP) at 25 ± 1 °C. Six reference substances were used to establish a regression line to derive the log Koc of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene. All substances were tested in duplicate. The retention times of each reference substance and the test item were measured to calculate the capacity factor (k) which was used to calculate the log Koc using the slope and intercept of regression line. A log Koc of 2.66 and 2.64 was calculated for the test substance resulting in a mean value of 2.65. Thus, the test item has a low potential for adsorption.