Reaction mass of Trisodium 4-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-{[1-ethyl-2-hydroxy-4-methyl-6-oxo-5-(sulfonatomethyl)-1,6-dihydropyridin-3-yl]diazenyl}benzenesulfonate and Trisodium 4-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-{[1-ethyl-2-hydroxy-4-methyl-6-oxo-5-(sulfonatomethyl)-1,6-dihydropyridin-3-yl]diazenyl}benzenesulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study completion date - 02 April 1984.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

CIBA Method A-11 - Comparable to internationally accepted guidelines.

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 40000/E
Purity: 67.1%

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 °C

pH:

10

Nominal and measured concentrations:

300 and 1000 mg/l

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At 300 mg/L: 10/10 fish survived at 96h interval.
At 1000 mg/L: 9/10 fish survived at 96h interval.
No mortality was observed at any time points.

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 of test item in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Executive summary:

An acute fish toxicity test was performed with Zebra-fish (Brachydanio rerio). This study was conducted according to method similar or equivalent to OECD test guideline 203.

10 fish per concentration were exposed at 300 and 1000 mg/L. Following deaths were observed.

At 300 mg/L: 10/10 fish survived at 96h interval.

At 1000 mg/L: 9/10 fish survived at 96h interval.

Based on the findings, the LC0 value was determined to be 300 mg/l. The LC50 and LC100 value was determined to be greater than 1000 mg/l.

Description of key information

The LC50 of FAT 40000 was determined to be greater than 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

A key study to determine acute toxicity to fish was performed with Zebra-fish (Brachydanio rerio). Groups of fish were exposed at the test concentrations of 300 and 1000 mg/L. Based on the findings, the LC0 values was 300 whereas LC50 and LC100 value was determined to be greater than 1000 mg/l. Several other studies conducted on different batches of this test material and involving different species confirmed this test result indication no species-dependent or production process and batch-dependent effects.

Overall result:

LC50 = >1000 mg/l.