Trichloroethylene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline study, some restrictions in reporting, nevertheless acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Effects on experimental animals of acute, repeated and continuous inhalation exposures to dichloroacetylene mixtures.

Author:

Siegel J, Jones RA, Coon RA, Lyon JP.

Year:

1971

Bibliographic source:

Toxicol Appl Pharmacol. 1971 Jan;18(1):168-74.

Materials and methods

Principles of method if other than guideline:

Determination of the LC50

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trichloroethylene

EC Number:

201-167-4

EC Name:

Trichloroethylene

Cas Number:

79-01-6

Molecular formula:

C2HCl3

IUPAC Name:

1,1,2-trichloroethene

Constituent 2

Reference substance name:

trichloroethene

IUPAC Name:

trichloroethene

Details on test material:

The substance was obtained commercially. No other details were reported.

Test animals

Species:

rat

Strain:

other: NMRI:O(SD) Sprague-Dawley derived

Sex:

male

Details on test animals or test system and environmental conditions:

No details were reported.

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

not specified

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Vapor of TCE was obtained in high concentrations by passing air through a large fritted gas absorber containing the material and then collected in a metallized plastic bag. All exposures were conducted under a fume hood. The animals were exposed for 4 hours and then returned to their individual cages for a 2-week observation period. No food or water was provided during the exposure.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

Gas chromatography equipped with a hydrogen flame ionisation detector. The chromatograph was equipped with an automatic valve which permitted sampling every 5 min during the exposure periods.

Duration of exposure:

4 h

Concentrations:

6750, 8000 and 14700 ppm.

No. of animals per sex per dose:

16

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no data
- Other examinations performed: no data

Statistics:

Not reported.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

12 500 ppm

Exp. duration:

4 h

Remarks on result:

other: Estimated by the method of Miller and Tainter (1944)

Mortality:

Mortality rate:
6750 ppm: 2/16
8000 ppm: 3/16
14700 ppm: 10/16

All deaths occurred during exposure.

Clinical signs:

other: Not reported.

Body weight:

Not reported.

Gross pathology:

Not reported.

Other findings:

Not reported.