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EC number: 701-241-0
CAS number: -
to the start of the study, the non-volatile component of the test item
was determined by adding a small, known amount of test item to glass
fibre filters and recording their weights. The filters were then dried
in a desiccator between 19 and 21°C for approximately 24 hours and then
weighed again. The difference in the two weights was taken as the
volatile content of the test item and the non-volatile component was
calculated as a percentage. The mean non-volatile component of the batch
used during the study was found to be ~0% (n=10). It was therefore
considered that a vapour study should be conducted and chemical analysis
should be employed in order to determine test atmosphere concentrations.
test atmosphere was sampled five times during the exposure period. The
sampling procedure involved 2 litres of test atmosphere being drawn
through two impingers containing acetonitrile (made up to 80ml). The
samples were then submitted for chemical analysis. The method of
analysis is given in attatched Appendix 10.
nominal chamber concentration was calculated by dividing the mass of
test item used by the total volume of air passed through the chamber.
The nominal concentration was similar to the actual concentration
showing the ease at which atmospheres could be generated from this test
1 Exposure Chamber Atmosphere Concentrations
Duration of Exposure (minutes)
Volume of Air Sampled (L)
Chamber Flow Rate (L/min)
Atmosphere Concentration (mg/L)
achieved atmosphere concentration (mg/L) =25.4
Air Flow (L/min)
Total Generation Time (mins)
Nominal Concentration (mg/L)
7 Temperature and Relative Humidity in Exposure Chamber
Chamber Temperature (ºC)
Chamber Relative Humidity (%) During Exposure
8 Air Flow and Oxygen Concentration in Exposure Chamber
Oxygen Concentration (%)
study was performed to assess the acute inhalation toxicity of the
test item. The method used followed that described in the OECD
Guidelines for Testing of Chemicals (2009) No. 403 “Acute Inhalation
Toxicity” and was designed to comply with Method B2 (Inhalation) of
Commission Regulation (EC) No. 440/2008, with the exception that only
six animals (three males and three females) were utilized during the
group of six HsdHanTM: WIST strain rats (three males and
three females) was exposed to a vapour atmosphere. The animals were
exposed for four hours using a nose only exposure system, followed by
a fourteen day observation period.
mean achieved atmosphere concentration was as follows:
Mean Achieved (mg/L)
The mortality data were summarised
Mean Achieved Atmosphere Concentration (mg/L)
Clinical Observations. Common
abnormalities noted during the study included increased respiratory
rate, ataxia, hunched posture, pilo-erection and wet fur. Animals
recovered such that males appeared normal on Day 4 post-exposure
females appeared normal one day later.
male animal exhibited a slight bodyweight loss on Days 0 – 1 and a
female exhibited a slight bodyweight loss on Days 0 – 3. Both animals
recovered to show normal bodyweight development during the remainder
of the recovery period.Normal
bodyweight development was noted for all other animals during the
course of the study.
macroscopic abnormalities were detected amongst animals at necropsy.
deaths occurred in a group of six rats exposed to a mean achieved
atmosphere concentration of 25.4 mg/L for four hours. It was therefore
considered that the acute inhalation median lethal concentration (4 hr
LC50) of Reaction Products of C3 alcohols and
C3 alkenes, in the HsdHanTM:
WIST strain rat, was greater than 25.4mg/L.
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