Copper chloride

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Copper Monochloride CAS No: 7758-89-6, Final 10/2006

Author:

OECD SIDS

Year:

2006

Reference 2

Reference Type:

other: Study cited in OECD SIDS

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)

GLP compliance:

yes

Type of assay:

other: Mammalian erythrocyte micronucleus test

Test material

Test material information

Constituent 1

Reference substance name:

Copper chloride

EC Number:

231-842-9

EC Name:

Copper chloride

Cas Number:

7758-89-6

Molecular formula:

Cl Cu

IUPAC Name:

λ¹-copper(1+) chloride

Details on test material:

- Name of test material (as cited in study report): Copper monochloride (CuCl),
- Analytical purity: purity 99%
- Lot/batch No.: Sigma-Aldrich corporation, LOT No. 17119BO

Test animals

Species:

mouse

Strain:

ICR

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data
- Age at study initiation: 8 weeks
- Weight at study initiation: no data

ENVIRONMENTAL CONDITIONS
- no data

Administration / exposure

Route of administration:

other: negative control: oral gavage; positive control: intraperitoneal injection

Vehicle:

- Vehicle(s)/solvent(s) used: 0.5 % CMC (carboxymethyl cellulose);
- Lot/batch no. (if required): LOT No.-32K0007

Details on exposure:

PREPARATION OF DOSING SOLUTIONS:
- no data

DIET PREPARATION
- no data

Duration of treatment / exposure:

single exposure

Frequency of treatment:

duplicate treatments (only positive control group was dosed once)

Post exposure period:

24 h

Doses / concentrations

Remarks:

Doses / Concentrations:
Three dose levels, 15, 30 and 60 mg/kg bw/day, were determined for the micronucleus experiment based on the results of dose range finding experiment
Basis:

No. of animals per sex per dose:

6

Control animals:

yes, concurrent vehicle

Positive control(s):

mitomycin C;
- Route of administration: intraperitoneal injection
- Doses / concentrations: 2.0 mg/kg of Mitomycin C

Examinations

Tissues and cell types examined:

polychromatic erythrocytes

Details of tissue and slide preparation:

CRITERIA FOR DOSE SELECTION:
dose range finding experiment

Evaluation criteria:

at least 1,000 polychromatic erythrocytes per animals were scored for the incidence of micronuclei.

Statistics:

Chi-square test (using a 2 × 2 contingency table)

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

valid

Negative controls validity:

not specified

Positive controls validity:

valid

Any other information on results incl. tables

All animals dosed with copper monochloride exhibited similar PCE/(PCE + NCE) ratios and MNPCE frequencies compared to those of negative control animals. All frequencies of MNPCE in the negative control groups fell
within acceptable ranges, while the positive control groups induced clear increase in the frequencies of MNPCE.