Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to the O.E.C.D. test guideline 202 with GLP compliance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report Date:
1983

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
other: Colorless liquid at room temperature.
Details on test material:
As per IUCLID5 Sections 1.1. 1.2. 1.4. and 4.1. for 2,3-epoxypropyl neodecanoate, REACH Registration: 01-2119431597-33-0000.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
As per IUCLID5 Sections 1.1. 1.2. 1.4. and 4.1. for 2,3-epoxypropyl neodecanoate, REACH Registration: 01-2119431597-33-0000.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
Quantities of stock solutions of the test substance in Analar acetone were added to triplicate sets of 140 ml glass flasks so that, when made up to 140 ml with a reconstituted fresh water, an approximately logarithmic series of concentrations ranging from 2—200 mg/L was produced. The final nominal concentrations were: 2, 4.3, 9.3, 20, 43, 93 and 200 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna, less than 24h old, were taken from a culture in STL derived from a strain obtained (via ICI Brixham Laboratory) from I.R.Ch.A., France The D. magna were cultured in glass vessels (0.5 to 4 litre) containing reconstituted fresh water. The cultures were held in a temperature controlled room, 20 ± 2°C, under a 16 h light 8 h dark cycle. The cultures were fed concentrated Chiorella vulgaris 0.5 mg/L of active dried yeast daily.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None.

Test conditions

Hardness:
The total hardness of the water was 190 mg/L as CaCO3
Test temperature:
The temperature of the test solutions was 20 ± 2°C.
pH:
pH was 8.1—8.3
Dissolved oxygen:
Concentration of dissolved oxygen was 8.8—9.2 mg/L
Salinity:
No data
Nominal and measured concentrations:
2, 4.3, 9.3, 20, 43, 93 and 200 mg/L nominal.
Details on test conditions:
A 48h static toxicity test was carried out without renewal of the test solutions. Quantities of stock solutions of 2,3-epoxypropyl neodecanoate in Analar acetone were added to triplicate sets of 140 ml glass flasks. Three flasks served as controls and received no test substance. The concentration of acetone in all test dishes, including the controls, was adjusted to 0.1 mL/L. Ten D. magna, less than 24h old, were allocated to each flask. In order to minimise the risk of D. magna becoming trapped at the surface, where at all concentrations tested, globules of test substance were visible, loosely fitting black paper caps were placed over the flasks to create a darkened zone which the D. inagna would avoid. After 24h and 48h the numbers of imiuobilised D. magna were counted and recorded. D. magna were considered to be immobile if, when the contents of the flask were briefly stirred they did not swim during a 10 second period of observation.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 4.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 4.0—5.7 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
At 4.3 mg.L 30% of the Daphnia were immobilized by 48 hr of treatment. At test substance concentrations of 9.3 mg/L and greater immobilization was generally 100% after 48 hr of exposure.
Reported statistics and error estimates:
The the 48 hr EC50 was 4.8 mg/L with 95% confidence limits 4.0—5.7 mg/L.

Any other information on results incl. tables

Globules of undissolved test substance were present on the surface of the water at all test concentrations. The test substance was therefore not wholly soluble at any of the concentrations tested.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48 hr EC50 value for the test substance to Daphnia magna was estimated to be 4.8 mg/L.
Executive summary:

The 48 hr EC50 of the test substance, 2,3-epoxypropyl neodecanoate to Daphnia magna was estimated by an O.E.C.D. test guideline 202 study. The 48 hr EC50 value for the test substance to Daphnia magna was estimated to be 4.8 mg/L. Therefore, it is anticipated that Neononanoic acid glycidyl ester will have a similar EC50 value under the conditions of the study.