4-tert-butylbenzaldehyde

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP guideline study. A solvent was used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38 412, L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

p-tert-butylaldehyde

IUPAC Name:

p-tert-butylaldehyde

Details on test material:

- Name of test material (as cited in study report): p-tertiaer-butylbenzaldehyd (TBA)
- Lot/batch No.: 932 B
- Analytical purity: 99.4 %
water solubility: 130 mg/L.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product dissolved in acetone (1.0 g/100 ml) was added to the test water; subsequently the fish were placed into the aquaria.
- Controls: solvent control containing 1.0 ml acetone/1 L test water was tested in parallel.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No undissolved test substance was visible during the test.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: golden Orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststadt, Germany
- Length at study initiation (length definition, mean, range and SD): 7.4 cm (range 6.7 - 8.4 cm)
- Weight at study initiation (mean and range, SD): 4.0 g (range 3.0 - 5.5 g)


ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, ad libitum.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): mortality0.3 %

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

19 - 20 °C

pH:

7.6 - 8.0

Dissolved oxygen:

7.5 - 8.8 mg/L

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
1.0,
2.15,
4.64,
10.0,
control,
solvent control

Details on test conditions:

TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm)
- Type (delete if not applicable): open / closed
- Fill volume: 10 l
- Aeration: Continuous aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 4.0 g fish/L test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/L CaCl2 * 2 H2O 123.3 mg/L MgSO4 + 7 H2O 63.0 mg/L NaHCO3 5.5 mg/L KCl
- Ca/mg ratio: 4:1


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 0, 4, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Results used to determine the conditions for the definitive study: LC 50 after 96hH : < 10 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

2.15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

4.64 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

48 h-LC50 of the positive control chloroacetamide was 32 mg/l, which corresponds to the normal sensitivity of the fish.
The 96 h results of 4-tert-butylbenzaldehyde to fish were as follows:
The LC50 was > 4.64 and < 10 mg/L (nominal).
The LC0 and the LC100 were 4.64 and > 10 mg/L (nominal), respectively.
The NOEC was 2.15 mg/L (nominal).

Reported statistics and error estimates:

Probit analysis (Finney 1971)

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to 4-tert-butylbenzaldehyde, mortalities were as follows:

Nominal test number of dead fish after x hours
concentration 1/4/24/48/72/96
(mg/l)
Control 0/0/0/0/0/0
Solvent control 0/0/0/0/0/0
1.00 0/0/0/0/0/0
2.15 0/0/0/0/0/0
4.64 0/0/1/1/1/1
10.00 0/0/4/6/7/7


Nominal test Symptoms of intoxication after x hours
concentration 1/4/24/48/72/96
(mg/L)
Control -/-/-/-/-/-
1.00 -/-/-/-/-/-
2.15 -/-/-/-/-/-
4.64 -/A/-/-/-/-
10.00 ANT/NT/NT/NT/N/N

Definitions of Symptoms:
-: No symptoms
+: All fish dead
N: Narcotic-like state
A: Apathy
T: Tumbling