2-butylaminoethanol

Specific investigations: other studies

Administrative data

Endpoint:

mechanistic studies

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Cholinesterase inhibition in the acute toxicity of alkyl-substituted 2-Aminoethanols

Author:

Hartung R, Cornish HH

Year:

1968

Bibliographic source:

Toxicology and applied pharmacology, 12: 486-494

Reference 2

Reference Type:

secondary source

Title:

Patty's Industrial Hygiene and Toxicology.

Author:

Clayton G, Clayton FE

Year:

1994

Bibliographic source:

4th. Ed., Vol. 2

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The acute oral and intraperitoneal toxicity of a series of alkyl-substituted 2-aminoethanols was studied in relation to cholinesterase inhibition. Cholinesterase inhibition was conducted in vitro (brain and red cells), using a purified enzyme preparation derived from bovine erythrocytes. The level of in vitro inhibition was related to the number of carbon atoms attached on the nitrogenous head of the 2-aminoethanol molecule.

GLP compliance:

no

Type of method:

in vitro

Endpoint addressed:

neurotoxicity

Test material

Test material information

Constituent 1

Reference substance name:

Butylethanolamine

IUPAC Name:

Butylethanolamine

Details on test material:

- Name of test material (as cited in study report): Butylethanolamine

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

not specified

Details on exposure:

no data

Duration of treatment / exposure:

no data

Frequency of treatment:

no data

Post exposure period:

no data

Doses / concentrations

Remarks:

Doses / Concentrations:
650-1210 mg/kg bw
Basis:

Details on study design:

The enzyme activity was analyzed by a modification of the electrometric method of Michel (1949). This modification consisted of a titration of the buffer system with dilute acetic acid, so that the change in pH could be related to the amount of acetic acid liberated by the enzymatic hydrolysis of the acetylcholine substrate. During the analysis the pH varied from 7.7 to 7.3 . The brain and red cell cholinesterase levels were determined in rats which were sacrificed 10-15 minutes after receiving an intraperitoneal dose of the test substance. The whole rat brains were removed immediately after sacrifice and homogenized with an equal volume of physiological saline at 0°C. Red cells were separated from the plasma by centrifugation and washed twice with physiological saline prior to analysis.

Results and discussion

Details on results:

The acute oral and intraperitoneal toxicity of a series of alkyl-substituted 2-aminoethanols was studied in relation to cholinesterase inhibition. All the 2-aminoethanols studied inhibited cholinesterase in vitro in brain and red cells of rats that were treated intraperitoneally.
The data of this publication indicate that neutralized N-alkyl substituted 2-aminoethanols inhibit cholinesterase in vitro. Inhibition increases as the degree of substitiution and the molecular size of the nitrogenous head of the aminoethanol molecular increase. When the concentrations of substituted aminoethanols producing 50% inhibition in vitro are compared with their intraperitoneal LD50 doses on a molar basis, these two variables were found to correlate well on a statistical basis: for the test substance an inhibiting concentration of 15 mmol/Liter was found and correlated to an oral LD50 of 61.9 and and i.p. LD50 of 7.17 mmol/kg. The oral LD50 was taken from previous studies.