Registration Dossier
Registration Dossier
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EC number: 422-630-9 | CAS number: 22208-25-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes
- Inoculum or test system:
- not specified
- Reference substance:
- other: Sodium acetate
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 41
- Sampling time:
- 28 d
- Details on results:
- Points of degradation plot (test substance):
0.5 % degradation after 2 d
3.3 % degradation after 7 d
5.6 % degradation after 9 d
13.6 % degradation after 14 d
32.5 % degradation after 23 d
40.3 % degradation after 29 d - Results with reference substance:
- Points of degradation plot (reference substance):
12.5 % degradation after 2 d
42.4 % degradation after 7 d
53.6 % degradation after 9 d
65.7 % degradation after 14 d
85.7 % degradation after 23 d
94 % degradation after 29 d - Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item degraded 41% after 28 days. The substance is not readily biodegradable.
- Executive summary:
The study was conducted according to EU Method C.4 (Carbon dioxide (CO2) evolution test) (GLP study). Sodium acetate was used as a reference substance. According to the results obtained, the substance was classified as not readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes
- Inoculum or test system:
- not specified
- Reference substance:
- other: Sodium acetate
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 40
- Sampling time:
- 28 d
- Details on results:
- Points of degradation plot (test substance):
1.3 % degradation after 4 d
5.5 % degradation after 9 d
13.5 % degradation after 14 d
32.4 % degradation after 23 d
37.8 % degradation after 27 d
40 % degradation after 29 d - Results with reference substance:
- Points of degradation plot (reference substance):
33.8 % degradation after 4 d
53.6 % degradation after 9 d
65.7 % degradation after 14 d
85.7 % degradation after 23 d
89.2 % degradation after 27 d
97.3 % degradation after 29 d - Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance degraded up to 40% after 28 days, being not readily biodegradable.
- Executive summary:
The study was performed according to EU Method C4 (Carbon dioxide evolution test (Modified Sturm Test)) (GLP study). The reference substance used was Sodium acetate. The results indicated that the test substance was degraded significantly (40% after 28d of incubation) during the test period. However, since at least 60% biodegradation was not reached within 10 days of biodegradation exceeding 10%, the criterion for ready biodegradability was not met.
Referenceopen allclose all
Description of key information
Key study: EU Method C4 (Carbon dioxide evolution test (Modified Sturm Test)) (GLP study). The biodegradation after 28 days was of 40%. The test item was determined not to be readily biodegradable.
Key study: EU Method C4 (Carbon dioxide (CO2) evolution test) (GLP study). The biodegradation after 28 days was of 41%. The test item was determined not to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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