Registration Dossier
Registration Dossier
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EC number: 200-449-4 | CAS number: 60-00-4
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Disodium dihydrogen ethylenediaminetetraacetate
- EC Number:
- 205-358-3
- EC Name:
- Disodium dihydrogen ethylenediaminetetraacetate
- Cas Number:
- 139-33-3
- Molecular formula:
- C10H14N2Na2O8
- IUPAC Name:
- disodium dihydrogen 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetate
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- A silty–loamy sand agricultural soil, USDA (Soil Survey Manual), was collected from 0 to 30 cm surface layer of the Melaten field in Aachen, Germany. The soil was air-dried at room temperature, sieved through a 2-mm sieve and characterised as follows:
pH: 6.8
organic matter: 3.5 %
soil fractions
sand: 49.4 %
Clay: 42.1 %
Silt: 8.5 %
Test organisms
- Species:
- other: Nicotiana tabacum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Tobacco plants (N. tabacum SR-1) were used for the experiment.
Study design
- Test type:
- vegetative vigour test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks:
- approximately 3 weeks
- Post exposure observation period:
- no data available
Test conditions
- Test temperature:
- 25/20 °C light/dark temperature regime
- pH:
- 6.40 - 6.83
- Moisture:
- 60% relative humidity
- Details on test conditions:
- 16 h light period (light intensity of 320 µmol/m2 * s)
- Nominal and measured concentrations:
- Nominal: 0.125,0.25, 1.25, 62.5, 125, and 250 mmol/kg for Na2EDTA
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Species:
- other: Nicotiana tabacum
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 84 - < 420 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Key result
- Species:
- other: Nicotiana tabacum
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.25 - < 1.25 other: mmol/kg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Details on results:
- EDTA did not show any adverse effects at a concentration of < 0.125 mmol/kg. At 1.25 mmol/kg of EDTA the leaves showed necrotic lesions and the dry weight was negligible. At a concentration of 0.25 mmol/kg EDTA the dry weight is only slightly lower but the standard deviation is higher in comparison to the 0.125 mmol/kg application.
- Results with reference substance (positive control):
- no data available
- Reported statistics and error estimates:
- no data availble
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the results of this vegetative vigour study EDTA had an inhibitory effect on growth of Nicotiana tabacum at rate of 1.25 mmol/kg soil (equivalent to 420 mg/kg). The NOEC will be determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil).
- Executive summary:
The effects of EDTA (Na2EDTA) on vegetative vigour of plants following application were examined in a laboratory test on Nicotiana tabacum. The test item was applied at a dose rate of up to 250 mmol/kg and compared to an untreated control.
According to the results, the test item had an inhibitory effect on growth of the tested species at rates of 1.25 mmol/kg soil (equivalent to 420 mg/kg) and thus determined to be the overall LOEC for this species.
The overall NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg).
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