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Based on the availble data the effects on terrestrial organisms are determined for EDTA (acid form) and its salts, for justification for read-across see IUCLID 5, Chapter 13. A LC50 -value derived from a 14 days acute test for earthworm was determined to be 158 mg/kg dry weight soil (Edwards, 2009). According to Evangelou et al. (2006) EDTA did not show adverse effect on vegetative vigour of Nicotiana tabacum up to and including an rate of 84 mg/kg soil. An ECx could not be calculated.

Due to the water solubility and the hydrophilic character of the substance a binding to the solid soil phase is negligible. Hence EDTA will be preferable expected in the pore water. It could be shown that the substance is enhanced biodegradable in water (see chapter 5.2.1). Therefore no further tests on terrestrial microorganisms are provided.