1-[(2-chloro-4-nitrophenyl)azo]-2-naphthol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18.01. – 13.02.2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: Test No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters."

Deviations:

yes

Remarks:

see Any other information

Principles of method if other than guideline:

- Principle of test:
The test item is almost insoluble thus a sample of the test item at a concentration of 0.1 g/L was dispersed in the dilution water and partly dissolved by using ultrasonic device. The total volume for one dilution was 1000 ml for preliminary and limit test.
Organisms: 3 in preliminary and 10 in limit test

- Short description of test conditions:
The dilution water should have a pH of ca 7.8 and saturated with oxygen at a temperature 20 – 24°C.
Test duration 96 hours, the temperature of the solutions 21.0 ± 1.0 °C.

- Parameters analysed / observed:
mortality at 48 and 96 h

GLP compliance:

no

Specific details on test material used for the study:

Other name: Versalová červeň 2G

Analytical monitoring:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test item is almost insoluble thus a sample of the test item at a concentration of 0.1 g/L was dispersed in the dilution water and partly dissolved by using ultrasonic device.
- Controls: dilution water
- Concentrations: 100, 75, 50, 25, 0 mg/L
- Evidence of undissolved material: yes

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Guppy
- Strain: Poecilia reticulata
- Source: AKVA-SALON, Žižkova 977, 535 01 Přelouč
- Age at study initiation (mean and range, SD): 3 - 4 monts
- Length at study initiation (length definition, mean, range and SD): 2 - 3 cm

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions (same as test or not): same as test

FEEDING DURING TEST
no feeding during test
fasted 72 hours before test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.0 ± 1.0 °C

pH:

7.77 - 7.99 (for measured values see attached document with tables)

Dissolved oxygen:

8.00 - 8.5 mg/L O2 (see attached document with tables)

Nominal and measured concentrations:

100.8 mg.L-1

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: open 10 L glass aquarium
- Aeration: without
- No. of organisms per vessel:
Preliminary test: 3 animals
Full test: 10 animals
- No. of vessels per concentration/control (replicates): no replicates

TEST MEDIUM / WATER PARAMETERS
Dilution water: standard dilution water was prepared by mixing 2.5 ml of stock solutions (A,B,C,D) with 1000 ml distilled water
A) 117.6 g CaCl3.2H2O in 1L of distilled water
B) 49.3 g MgSO4.7H2O in 1L of distilled water
C) 25.9 g NaHCO3 in 1L of distilled water
D) 2.3 g KCl in 1L of distilled water
The dilution water should have a pH of ca 7.8 and is saturated with oxygen at a temperature 20 – 24°C.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 48, 96 h

TEST CONCENTRATIONS
- Range finding study: 100, 75, 50, 25 mg.L-1
- Test concentrations: 100.8 mg.L-1

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

The sensitivity of the test organisms and the correctness of the test were verified by testing a standard substance (potassium dichromate p.a.).

Validity criteria fulfilled:

yes

Remarks:

The measured values of pH solution not change more than 1 pH unit.

Conclusions:

48 h - LC50 > 100.0 mg.L-1
96 h - LC50 > 100.0 mg.L-1

Executive summary:

The test item, Versalová červeň 2G (Pigment Red 4), was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 > 100.0 mg.L-1

96 h - LC50 > 100.0 mg.L-1

Description of key information

The test substance was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 > 100.0 mg.L-1

96 h - LC50 > 100.0 mg.L-1

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L