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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study was conducted according to the appropriate OECD Test Guideline and a statement of GLP compliance is included in the test report.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Polyol TD
- Physical state: Clear, colourless liquid

- Composition of test material, percentage of components:
5-ethyl-5-hydroymethyl-1,3-dioaxane (CAS number 5187-23-5, EINECS number 225-967-8): 25 - 60%
1,3-Propandiol, 2-ethyl (CAS number 2612-29-5, EINECS number 220-08-3): 20 - 50%
2-ethyl-2-hydroxymethyl-1,3-propanediol (CAS number 77-99-6, EINECS number 201-074-9): 15 - 25%


- Lot/batch No.: 3777937
- Expiration date of the lot/batch: Stable for at least 6 months.
- Storage condition of test material: Sealed containers, room temperature, dark.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples of the stock solution and the highest tested concentration at 0 h and 72 h were collected and stored in the freezer until analysed by the
Sponsor.

Test solutions

Vehicle:
no
Details on test solutions:
2000.1 mg of the test item was solved in 1000 mL of growth medium by magnetic stirring on the day of the test start. The test solutions were made by dilution of the stock solution (2000 mg/L) with growth medium.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: NIVA CHL 1
- Source (laboratory, culture collection): Toxicon's culture, originating from NIVA, Oslo, Norway.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
Not applicable
Test temperature:
21 - 24ºC - nominal values
pH:
8.1±0.2 - nominal values
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
nominal: 31.2, 62.5, 125, 250, 500, 1000, 2000 mg/L.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): E-bottles
- Material, size, headspace, fill volume: glass, 250 mL, 100 mL fill volume
- Aeration: not applicable
- Control end cells density: Yield (end density - starting density) = 556667 cells/mL (C.V. = 16.1%).
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6



GROWTH MEDIUM
- Standard medium used: yes (as specified in OECD TG 201)


OTHER TEST CONDITIONS
- Sterile test conditions: yes (components of growth medium were autoclaved or sterile filtered before use.
- Adjustment of pH: No.
- Photoperiod: Continuous illumination
- Light intensity and quality: 100 µEm-2s-1


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: The number of cells/mL was counted by microscope at 0, 24, 48, and 72 hours.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2-fold ( 31.2, 32.5, 125, 250, 500, 1000, and 2000 mg/L)
Reference substance (positive control):
yes
Remarks:
Test substance not identified

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
743 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
62 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
144 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
62 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
- Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: No
Results with reference substance (positive control):
Report states the following:

A qualification test on the alga culture was made on 5 June 2008 to assure the quality of the test animals. ErC50 was 1.5 mg/L which confirmed theculture was suitable as a test organism according to OECD TG no 201.
Reported statistics and error estimates:
The EC50 values were calculated by means of graphic interpolation. The NOEC was defined as the highest tested concentration not statistically different from the controls according to the Mann-Whitney U-test (p>0.05)

Any other information on results incl. tables

Calculated specific growth and biomass results (72 hours)

Nominal concentration (mg/L)

72 hours Specific growth rate (μ) Mean ± CV (%)

% inhibition

Control

1.34 ± 4.0

-

31.2

1.27 ± 5.8

5

62.5

1.19 ± 13.0

11

125

1.11 ± 16.0

17

250

1.03 ± 5.8

23

500

0.97 ± 6.2

28

1000

0.35 ± 26.8

74

2000

0.0

100

 

72 h Yield (cells/ml)

Mean ± CV (%)

% inhibition

Control

556667 ± 16.1

-

31.2

450000 ± 21.2

19

62.5

373333 ± 40.2

33

125

290000 ± 45.6

48

250

213333 ± 17.8

62

500

176667 ± 18.2

68

1000

2000 ± 50.0

100

2000

0.0

100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A 72 hour algal growth inhibition study, test species Selenastrum capricornutum, was performed to determine the toxic effects of the test substance Polyol TD. The test was conducted according to OECD test guideline 201, and performed to GLP. Based on nominal exposure concentrations the 72 hour ErC50 was calculated to be 743 mg/L and the corresponding no-observed effect concentration (NOEC) was 62 mg/L. The 72 hour EyC50 was calculated to be 144 mg/L and the corresponding NOEC was 62 mg/L.
Executive summary:

A 72 hour algal growth inhibition study, test species Selenastrum capricornutum, was performed to determine the toxic effects of the test substance Polyol TD. The test was conducted according to OECD test guideline 201, and performed to GLP. Based on nominal exposure concentrations the 72 hour ErC50 was calculated to be 743 mg/L and the corresponding no-observed effect concentration (NOEC) was 62 mg/L. The 72 hour EyC50 was calculated to be 144 mg/L and the corresponding NOEC was 62 mg/L.