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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Study Experimental phase 2002-09-20 to 2003-02-05. Report Date 2003-04-02
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
443-510-2
EC Name:
-
Cas Number:
738587-10-5
Molecular formula:
C26H14N10Ni2O18S4 as free acid
IUPAC Name:
dinickel(2+) sodium 3-carboxy-5-[(1E)-2-(7-oxido-2,6-disulfonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide 3-carboxy-5-[(1E)-2-(7-oxido-2-sulfo-6-sulfonatonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide
Radiolabelling:
no

Study design

Analytical monitoring:
yes
Buffers:
- pH 4.0 Buffer Solution
164ml of 0.2M acetic acid solution added to 36ml of 0.2M sodium acetate solution and diluted to 1 litre with distilled/deionised water. Measured pH = 4.01

- pH 7.0 Buffer solution
296ml of 0.1M sodium hydroxide solution added to 500ml of 0.1M monopotassium phosphate solution and diluted to 1 litre with distilled/deionised water. Measured pH = 7.05

- pH 9.0 Solution
0.1M sodium hydroxide solution, pH adjusted to 9 with 0.1M hydrochloric acid. Measured pH = 9.10

All buffer solutions were degassed with helium before use.
Details on test conditions:
Preparation of Test Solutions
The tests were prepared in duplicate in each buffer solution at around 0.5 g/L and placed in an oven at 50 +/-1 degree C. Analysis of the tests (by HPLC) was carried out initially and then over several days.
Duration of testopen allclose all
Duration:
7 d
pH:
4
Temp.:
50
Initial conc. measured:
5.13 g/L
Duration:
7 d
pH:
7
Temp.:
50
Initial conc. measured:
4.9 g/L
Duration:
7 d
pH:
9
Temp.:
50
Initial conc. measured:
5.36 g/L
Number of replicates:
2
Positive controls:
no
Negative controls:
no

Results and discussion

Preliminary study:
Less than 10% hydrolysis had occured at pH 4, 7 and 9 after five days storage at 50 +/-1 degree C therefore no further testing was carried out.
Transformation products:
no
Total recovery of test substance (in %)open allclose all
% Recovery:
98.1
pH:
4
Temp.:
50 °C
Duration:
7 d
% Recovery:
98.5
pH:
4
Temp.:
50 °C
Duration:
7 d
% Recovery:
100.2
pH:
7
Temp.:
50 °C
Duration:
7 d
% Recovery:
103.8
pH:
7
Temp.:
50 °C
Duration:
7 d
% Recovery:
101.1
pH:
9
Temp.:
50 °C
Duration:
5 d
% Recovery:
102.1
pH:
9
Temp.:
50 °C
Duration:
5 d
Details on results:
Less than 10% hydrolysis had occurred in the preliminary study at all three pH's after 5 days storage at 50°C ± 1 deg C therefore, no further testing was carried out.

Applicant's summary and conclusion

Conclusions:
In conclusion less than 10% hydrolysis had occurred in the preliminary study at pH 4, 7 and 9 after 5 days storage at 50°C ± 1 deg C.
Executive summary:

Introduction

This study was undertaken to assess the hydrolysis of the test substance at different pH values.

Results & Conclusions

After 5 days <10% hydrolysis occured at pH's 4, 7 and 9 therefore, no further work was carried out.