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EC number: 203-744-6 | CAS number: 110-18-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-10-05 to 2017-11-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Principles of method if other than guideline:
- N/A
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: TMEDA, termed ATMEDAHP in the report
Purity: 99.9%
Lot number: 17F-1069978
C content: 62.01% (calculated)
Appearance: Clear to slightly hazy, pale yellow liquid - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The test inoculum was provided by a sample of activated sludge obtained from AZV Staufener Bucht, a municipal STP (140,000 population equivalent) that receives predominantly domestic wastewater. The sample was obtained on the day before the start of the study and washed in tap water and test medium prior to use. Activated sludge was applied at a concentration of 30 mg dry solids/L in each of the test vessels.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The test design comprised the following treatments:
3 x test vessels containing TMEDA (20 mg TOC-equiv/L);
3 x reference vessels containing Na benzoate (20 mg TOC-equiv/L);
1 x toxicity control vessel containing TMEDA + Na benzoate (each at 20 mg TOC-equiv/L)
3 x blank control vessels (inoculated test medium, no other addition). - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- Not required.
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 60
- Sampling time:
- 28 d
- Remarks on result:
- other: not readily biodegradable based on test result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: No degradation in 2 of 3 replicates throughout the test.
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 36.5
- Sampling time:
- 28 d
- Remarks on result:
- other: Significant late-onset degradation in replicate 3 began between D21 and D28
- Details on results:
- Disparate degradation of TMEDA was observed in the triplicate vessels containing the test substance. No degradation (negative degradation, implying CO2 yield below the background recorded in the blanks) was observed in two of the vessels throughout the entire incubation. The third replicate showed a similar lack of CO2 production up to and including Day 21, but abruptly reached 32.9% ThCO2 yield on Day 28 and 36.5% ThCO2 after terminal acidification, indicating late-onset biodegradation during the final week of the test. Since measured CO2 yield did not reach 60% ThCO2 within 28 days, it may be concluded that TMEDA is not readily biodegradable.
Given the timing of the onset of CO2 production in the third replicate, it is not possible to determine whether biodegradation had reached a plateau by the end of the incubation, or whether it was still in progress at the time when biological activity was stopped by acidification. However, the indication of significant biodegradation in the third replicate does suggest that more extensive and consistent degradation might occur under extended incubation conditions. TMEDA may be considered to be inherently biodegradable, not fulfilling specific criteria. - Results with reference substance:
- Mean CO2 production in the triplicate reference vessels containing sodium benzoate exceeded 60% within 4 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable, not fulfilling specific criteria
- Conclusions:
- The ready biodegradability of TMEDA was determined according to OECD 301B (CO2 Evolution Test), in compliance with GLP. Mean measured CO2 production after 28 days was below 60% of the theoretical yield and TMEDA is therefore not classifiable as readily biodegradable. However, the indication of significant biodegradation in the third replicate does suggest that more extensive and consistent degradation might occur under extended incubation conditions. TMEDA may be considered to be inherently biodegradable, not fulfilling specific criteria.
- Executive summary:
The ready biodegradability of TMEDA was determined in a GLP-compliant test performed according to OECD 301B (CO2 Evolution Test). The test substance was dissolved at a concentration equivalent to 20 mg organic carbon/L in a mineral salts medium inoculated with activated sludge from a STP treating domestic wastewaters, applied at a concentration of 30 mg suspended dry solids/L. Three test vessels were assigned to the test substance. A further three vessels were used for the readily biodegradable reference substance (Na benzoate, also applied at 20 mg organic C-equiv/L) and three more vessels were used for the blank control containing only inoculated test medium. A further single vessel contained TMEDA combined with Na benzoate (each applied at 20 mg organic C-equiv/L) and served as a toxicity control to check for potential inhibition of microbial activity caused by the test substance. All vessels were sealed and continually purged throughout the 28 -d incubation with a flow of CO2 -free air, with the exhaust from each vessel passing through a dedicated absorber wash bottle containing NaOH solution to trap evolved CO2. Samples of the absorber solution were taken at intervals during the incubation and analysed to determine inorganic carbon concentrations and hence the quantity of CO2 evolved from each vessel. Vessels were acidified on day 28 and the air-purge continued for a further 24 h to release any residual CO2 held in the test media. CO2 yields, corrected by subtracting contemporary mean background CO2 production in the blank vessels, were expressed as % theoretical CO2 (ThCO2) yield to indicate the extent of biodegradation.
Disparate degradation of TMEDA was observed in the triplicate vessels containing the test substance. No degradation (negative degradation, implying CO2 yield below the background recorded in the blanks) was observed in two of the vessels throughout the entire incubation. The third replicate showed a similar lack of CO2 production up to and including Day 21, but abruptly reached 32.9% ThCO2 yield on Day 28 and 36.5% ThCO2 after terminal acidification, indicating late-onset biodegradation during the final week of the test. Since measured CO2 yield did not reach 60% ThCO2 within 28 days, it may be concluded that TMEDA is not readily biodegradable. Given the timing of the onset of CO2 production in the third replicate, it is not possible to determine whether biodegradation had reached a plateau by the end of the incubation, or whether it was still in progress at the time when biological activity was stopped by acidification. However, the indication of significant biodegradation in the third replicate does suggest that more extensive and consistent degradation might occur under extended incubation conditions. TMEDA may therefore be considered to be inherently biodegradable, not fulfilling specific criteria.
The reference substance Na benzoate achieved the criteria for classification as readily biodegradable within 4 days, which confirms the activity of the inoculum. CO2 production in the toxicity control exceeded 25% of total ThCO2 within 14 days, which may be taken to signify that TMEDA dosed at 20 mg organic C-equiv caused no inhibition of microbial activity that might account for the limited degree of biodegradtion observed under the conditions of the test.
Reference
Table 5.2.1 -1: Biodegradation of TMEDA under ready biodegradability (OECD 301B; CO2 Evolution) test conditions
Day |
Biodegradation: blank-corrected CO2 yield as % of theoretical |
||||||
TMEDA |
Na benzoate reference |
Toxicity control |
|||||
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
‑3.4 |
‑1.2 |
‑2.4 |
60.8 |
72.7 |
67.4 |
33.5 |
7 |
‑4.7 |
‑1.0 |
‑3.8 |
74.7 |
81.9 |
81.4 |
37.7 |
11 |
‑7.7 |
‑2.0 |
‑6.6 |
80.4 |
82.2 |
83.9 |
38.6 |
14 |
‑8.8 |
‑2.3 |
‑8.4 |
81.6 |
88.1 |
86.1 |
38.8 |
21 |
‑17.0 |
‑2.7 |
‑16.1 |
79.4 |
91.3 |
85.9 |
35.2 |
28 |
‑2.0 |
‑4.7 |
32.9 |
81.8 |
92.0 |
92.0 |
31.0 |
(29)a |
‑21.4 |
‑2.6 |
36.5 |
79.8 |
92.0 |
90.4 |
33.1 |
a Includes final purge of CO2following acidification of test media and cessation of biological activity on D 28. |
Description of key information
The ready biodegradability of TMEDA was determined in a GLP-compliant test performed according to OECD 301B (CO2 Evolution Test).
Disparate degradation of TMEDA was observed in the triplicate vessels containing the test substance. No degradation (negative degradation, implying CO2 yield below the background recorded in the blanks) was observed in two of the vessels throughout the entire incubation. The third replicate showed a similar lack of CO2 production up to and including Day 21, but abruptly reached 32.9% ThCO2 yield on Day 28 and 36.5% ThCO2 after terminal acidification, indicating late-onset biodegradation during the final week of the test. Since measured CO2 yield did not reach 60% ThCO2 within 28 days, it may be concluded that TMEDA is not readily biodegradable.
Given the timing of the onset of CO2 production in the third replicate, it is not possible to determine whether biodegradation had reached a plateau by the end of the incubation, or whether it was still in progress at the time when biological activity was stopped by acidification. However, the indication of significant biodegradation in the third replicate does suggest that more extensive and consistent degradation might occur under extended incubation conditions. TMEDA may be considered to be inherently biodegradable, not fulfilling specific criteria.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, not fulfilling specific criteria
- Type of water:
- freshwater
Additional information
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