Dimethyl vinylphosphonate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 September 1984 and 28 September 1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Version / remarks:

1981

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl vinylphosphonate

EC Number:

225-076-4

EC Name:

Dimethyl vinylphosphonate

Cas Number:

4645-32-3

Molecular formula:

C4H9O3P

IUPAC Name:

Dimethyl ethenylphosphonate

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: HOECHST AG, Kastengrund
- Weight at study initiation: 3.0 - 4.2 kg
- Housing: individually
- Diet: ad libitum (Altromin 2123)
- Water: ad libitum (deionised, chlorated water)

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 2
- Humidity (%): 55 +/- 10
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 0.1 mL

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

1, 24, 48 and 72 hours after application

Number of animals or in vitro replicates:

3 animals

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: Yes (physiological saline solution)
- Time after start of exposure: 24 hours

SCORING SYSTEM: according to Draize

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 2

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 3

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.7

Max. score:

4

Reversibility:

fully reversible within: 72 hours

Results 4

Irritation parameter:

iris score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

2

Results 5

Irritation parameter:

iris score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

2

Results 6

Irritation parameter:

iris score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.7

Max. score:

2

Reversibility:

fully reversible within: 72 hours

Results 7

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

3

Results 8

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

3

Results 9

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1.3

Max. score:

3

Reversibility:

fully reversible within: 72 hours

Results 10

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 11

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 12

Irritation parameter:

chemosis score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 72 hours

Irritant / corrosive response data:

- 1 hour after application: Animal 3 showed strong chemosis (score 3). In one animal, the vessels of the conjunctivae were clearly injected, in the second animal there was a diffuse, crimson redness. Hyperemia of the iris appeared, as well as slight corneal opacity occured occasionally.
- 24 hours after application: Two animals were symptom-free. One animal showed marked swelling, as well as diffuse fleshy redness at the conjunctivae. Furthermore, a hyperemia of the iris and a slight corneal opacity was observed. The symptoms were accompanied by whitish discharge.
- 72 hours after application: All animals were symptom-free.

Other effects:

- Ophthalmoscopic findings: none

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The test item is not considered to be irritanting to rabbits' eye according to OECD TG 405.

Executive summary:

The acute eye irritation study of the test item was performed in three New Zealand White rabbits according to OECD 405. The irritation effect of the test item was evaluated according to the Draize method. A single dose of 0.1 mL of the test item was placed into the conjunctival sac of the left eye of each animal. The untreated right eye served as control. The treated eyes of the test animals were washed out 24 hours after application. The eyes were examined at 1, 24, 48, and 72 hours after the application.

One hour after application animal 3 showed strong chemosis (score 3). In one animal, the vessels of the conjunctivae were clearly injected, in the second animal there was a diffuse, crimson redness. Hyperemia of the iris appeared, as well as slight corneal opacity occured occasionally. 24 hours after application two animals were symptom-free. One animal showed marked swelling, as well as diffuse fleshy redness at the conjunctivae. Furthermore, a hyperemia of the iris and a slight corneal opacity was observed. The symptoms were accompanied by whitish discharge.

All animals were symptom-free 72 hours after application.

For animal 1 and 2, the mean scores (considering readings at 24, 48 and 72 hours after test item application) were 0 for cornea opacity, 0 for iris, 0 for conjunctivae redness and 0 for chemosis. For animal 3, the mean scores were 0.7 for corneal opacity, 0.7 for iris, 1.3 for conjunctivae redness and 1.0 for chemosis. Therefore, the test item is not considered to be irritating to rabbits’ eye.