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EC number: 237-525-1 | CAS number: 13826-35-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Data is from online Chemical Repository Database
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- Salmonella/microsomal assay to investigate the mutagenic potentlaI of a test compound 3-Phenoxybenzyl alcohol (3-PBA)
- GLP compliance:
- not specified
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- 3-phenoxybenzylic alcohol
- EC Number:
- 237-525-1
- EC Name:
- 3-phenoxybenzylic alcohol
- Cas Number:
- 13826-35-2
- Molecular formula:
- C13H12O2
- IUPAC Name:
- (3-phenoxyphenyl)methanol
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): 3-Phenoxybenzyl alcohol
- Molecular formula (if other than submission substance): C13H12O2
- Molecular weight (if other than submission substance): 200.23318 g/mol
- Smiles notation (if other than submission substance): C1=CC=C(C=C1) OC2=CC=CC (=C2) CO
- InChl (if other than submission substance): InChI=1S/C13H12O2/c14-10-11-5-4-8-13(9-11)15-12-6-2-1-3-7-12/h1-9, 14H, 10H2
- Substance type: Organic
- Physical state: liquid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA1535, TA100, TA1537, TA1538 and TA98
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Spizzizen's minimal medium ,Noble's agar,Nutrient broth
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 from Sprague-Dawley rats
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- other: N-methyl- N -nitro-N-nitrosoguanidine,2-Aminoanthracene,2-Aminofluorene, 6-Aminochrysene, Aflatoxin B1
- Details on test system and experimental conditions:
- METHOD OF APPLICATION :in agar (plate incorporation)
- Evaluation criteria:
- significant increase in the number of revertant mutant colonies when compared to the spontaneous background rate which occurs in the control cultures
- Statistics:
- Linear regression analysis to determine strength of dose response
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA1535, TA100, TA1537, TA1538 and TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
RESULTS OF SALMONELLA/MICROSOMAL ASSAY WITHOUT METABOLIC ACTIVATION ON 3-PBA
compound |
conc units/ plate |
REVERTANTS PER PLATE OF BACTERIAL TESTER STRAIN TA1535 TA1537 TA1538 TA100 TA98 |
||||
bacteria only (negative control) |
100.0000 µL |
7.0 ± 2.0 |
5.3 ± 1.2 |
9.7 ± 1.5 |
126.7 ± 10.4 |
15.3 ± 5.5 |
DMSO (SOLVENT CONTROL) |
100.0000 µL |
9.0 ± 2.6 |
5.3 ± 1.5 |
8.7 ± 3.5 |
139.0 ± 24.2 |
16.0 ± 1.0 |
MNNG (POSITIVE CONTROL) |
5.0000 µg |
813.7 ± 66.2 |
NT |
NT |
855.3 ± 203.3 |
NT |
DMSO (POSITIVE CONTROL) |
100.0000 µg |
NT |
463.3 ± 62.4 |
NT |
NT |
NT |
DMSO (POSITIVE CONTROL) |
5.000 µg |
NT |
NT |
264.3 ± 110.6 |
NT |
271.3 ± 40.4 |
3-PBA |
.0050 µL |
11.3 ± 2.1 |
5.0 ± 2.0 |
4.0 ± 1.0 |
229.3 ± 29.3 |
15.0 ± 3.5 |
0.5 % SOLUTION (v/v) IN DMSO |
.0100 µL |
16.3 ± 3.2 |
3.7 ± 2.1 |
4.0 ± 1.0 |
187.7 ± 18.6 |
15.3 ± 4.5 |
.050 0 µL |
16.7 ± 2.5 |
6.0 ± 2.6 |
6.7 ± 3.1 |
201.7 ± 26.8 |
16.3 ± 2.5 |
|
.1000 µL |
13.7 ± 1.5 |
6.3 ± .6 |
3.3 ± 2.5 |
189.7 ± 7.8 |
17.7 ± 2.3 |
|
.5000 µL |
10.3 ± 1.5 |
1.7 ± 0.6 |
6.0 ± .0 |
155.3 ± 21.4 |
17.7 ± 2.1 |
NT: not tested
RESULTS OF SALMONELLA/MICROSOMAL ASSAY WITH METABOLIC ACTIVATION ON 3-PBA
compound |
conc units/ plate |
Revertants per plate of bacterial tester strain TA1535 TA1537 TA1538 TA100 TA98 |
||||
Bacteria only (negative control) |
100.0000 µL |
7.0 ± 2.0 |
5.3 ± 1.2 |
9.7 ± 1.5 |
126.7 ± 10.4 |
15.3 ± 5.5 |
S-9 FRACTION(NEGATIVE CONTRL) |
100.0000 µL |
8.3 ± .6 |
5.3 ± 0.6 |
9.3 ± 2.3 |
131.7 ± 41.5 |
21.7 ± 3.1 |
DMSO( SOLVENT CONTRL) |
100.0000 µL |
9.7 ± 2.5 |
6.3 ± 1.2 |
11.3 ± 3.8 |
126.3 ± 38.1 |
17.3 ± 8.4 |
2-AA(POSITIVE CONTROL) |
5.0000 µg |
74.0 ± 10.1 |
NT |
NT |
NT |
NT |
6-AC(POSITIVE CONTROL) |
1.000 µg |
NT |
51.3 ± 7.1 |
NT |
NT |
NT |
2-AF(POSITIVE CONTROL) |
2.000 µg |
NT |
NT |
256.7 ± 64.5 |
NT |
NT |
AFLTOXIN (POSITIVE CONTROL) |
1.000 µg |
NT |
NT |
NT |
656.3 ± 203.9 |
307.0 ± 66.8 |
3-PBA 0.5 % SOLUTION (V/V) IN DMSO |
.0050 0 µL |
14.7 ± 2.3 |
13.3 ± 2.1 |
18.7 ± 5.0 |
280.3 ± 18.6 |
18.7 ± 5.0 |
.01000 µL |
8.3 ± 1.5 |
16.7 ± 4.0 |
13.0 ± 1.0 |
245.7 ± 4.5 |
22.3 ± 8.5 |
|
.05000 µL |
7.0 ± 0.0 |
12.0 ± 2.6 |
15.3 ± 1.5 |
243.7 ± 16.8 |
23.3 ± 3.5 |
|
.1000 µL |
12.0 ± 4.6 |
13.3 ± 5.5 |
11.7 ± 2.5 |
216.0 ± 10.4 |
20.0 ± 7.5 |
|
.5000 µL |
12.3 ± 4.2 |
10.0 ± 1.0 |
5.7 ± 2.1 |
175.0 ± 39.9 |
12.0 ± 2.6 |
NT: not tested
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The genetic toxicity test for 3-Phenoxybenzylalcohol (CAS No: 13826-35-2) on salmonella strains TA1535, TA100, TA1537, TA1538 and TA98 was found to be negative with and without activation. - Executive summary:
Genetic toxicity test was performed by using 3-Phenoxybenzylalcohol onsalmonella strainsTA1535, TA100, TA1537, TA1538 and TA98 with and without metabolic activation.Liver S-9 preparations were done from Sprague-Dawley male rats pretreated with Aroclor 1254 for the non-specific induction of hepatic enzymes served as the activation system. S-9 preparation was used at a final concentration of 100 µl per ml of S-9 mix. Dimethyl sulphoxide (DMSO) was the solvent used. Spizzizen's minimal medium, Noble's agar, Nutrient broth were the medium used in these experiments.
The test was performed according to the method of Ames et al.For the assay, the following were added, in order to 16 x 125 mm screw-capped tubes:
1.2 ml molten agar (0.6% Noble's agar supplemented with 0.6% NaCl , 0.5 mM biotin, and 0.5 mM histidine).
2.0.1 ml of bacterial culture.
3.0.1 ml of test agent in the appropriate solvent.
Tubes were mixed quickly but gently and the contents poured over a base plate of Spizzizen's minimal medium. Plates were rotated to distribute the overlay agar, allowed to harden and incubated at 370C for 48 hr at which time the number of mutant colonies were determined.
In case of metabolic activation with S-9, all procedure was same except that 0.5 ml of S-9 mix was added to the overlay agar immediately before it was mixed and poured. Plates were incubated.
Each agent was tested at five concentrations against all five strains of bacteria.
The following controls were included in each experiment:
Bacteria only for spontaneous reversion (Negative control), Bacteria and solvent for solvent control.Chemical used as positive control:Without metabolic activation areN-methyl- N -nitro-N-nitrosoguanidine, 2-Nitrofluorene, 9-Aminoacridineandwith metabolic activation:2-Aminoanthracene, 2-Aminofluorene, 6-Aminochrysene, Aflatoxin B1.
The test agent did not induce a significant increase in the number of point mutations inSalmonella typhimuriumstrains in the absence and in the presence of the activating system for strains TA1535, TA1537, TA1538, TA100, and TA98.
So from the above test procedure it was concluded thatthe genetic toxicity test for 3-Phenoxybenzylalcohol (CAS No: 13826-35-2) onsalmonellastrains TA1535, TA100, TA1537, TA1538 and TA98 was found to be negative with and without activation.
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