Diallyl carbonate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 14, 2001 to August 6, 2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non GLP-compliant screening study according to (not specified) OECD test guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Version / remarks:

screening test

Deviations:

yes

Principles of method if other than guideline:

A 0.1 ml aliquot of the supplied test item was introduced into the right eye of a single animal by gently pulling away the lower lid from the eyeball to form a cup into which the test item was placed. The lids were then held shut for a few seconds to prevent loss of the test item. The left eye remained untreated. Approximately 1, 24, 48 and 72 hours and 7 days after dosing the treated eye was examined for damage or irritation to the cornea, iris and conjunctivae using the untresated eye as a comparator control. The observed irritation was allocated a numerical value based on the Draize-scale.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diallyl carbonate

EC Number:

239-106-9

EC Name:

Diallyl carbonate

Cas Number:

15022-08-9

Molecular formula:

C7H10O3

IUPAC Name:

bis(prop-2-en-1-yl) carbonate

Details on test material:

- Name of test material (as cited in study report): DAC
- Physical state: colourless liquid
- Lot/batch No.: 011009
- Expiration date of the lot/batch: October 9, 2002
- Storage condition of test material: ambient conditions
- Other: RTC reference number 5979

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Test system

Controls:

no

Duration of treatment / exposure:

The lids were then held shut for a few seconds to prevent loss of the test item.

Observation period (in vivo):

1 - 72 hours and 7 days after application

Number of animals or in vitro replicates:

one

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Remarks:

cornea, iris and conjunctivae

Basis:

animal #1

Remarks:

screening test, one animal only

Time point:

other: 1, 24, 48 and 72 hours; 7 days

Score:

0

Max. score:

0

Reversibility:

fully reversible

Any other information on results incl. tables

Animal number: 37		Body weight (kg): 2.5
Region of eye	Parameter	Ocular reaction
		hours
		1	24	48	72
conjunctivae	Discharge:	0	0	0	0
	Chemosis:	0	0	0	0
	Redness:	0	0	0	0
Iris	Inflammation:	0	0	0	0
Cornea	Opacity:	0	0	0	0
	Area:	0	0	0	0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The results indicate that the test item has no irritating effect in the eye of the rabbit.