Diallyl carbonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 14, 2001 to August 6, 2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non GLP-compliant screening study according to (not specified) OECD test guidelines principles and procedures of the GLP-regulations 1999/11/EC and Italian Decreto Legislativo 27 Gennaio 1992 n. 120

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

screening test

Deviations:

not applicable

Principles of method if other than guideline:

Daphnia magna from the test facility's own colony were used. Daphnids were maintained in ELENDT M7 medium with the unicellular green alga Pseudokirchneriella subcapitata (Sleneastrum capricornutum) avaialbe as a food source.
The test item was solubilized in ELENDT M7 test medium at concentrations of 1, 10 and 100 mg/l.
Daphnids which were less than 24 hours old and of normal appearance and viability were selected for use. Animals were placed into 4 groups, each of 10 animals and divided into 2 sub-groups of 5 animals.
One group acted as control, three groups were treated with the test item at exposure levels of 1, 10 and 100 mg/l. All containers were inspected 24 and 48 hours after dosing. Immobilisation was considered to be occured if the animals were not capable of swimming within 15 seconds after gentle agitation of the container. The warter in all containers was monitored before start of the test and at the end of the test. Oxygen concentrations, temperature and ph were measured and recorded.
The test was regarded valid of immobilisation of controls did not exceed 10 % at the end of the test; the dissolved oxygen concnetration was greater than 2 mg/l throughout the period of the test and the pH did not change by more than 1.5 units during the period of the test.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diallyl carbonate

EC Number:

239-106-9

EC Name:

Diallyl carbonate

Cas Number:

15022-08-9

Molecular formula:

C7H10O3

IUPAC Name:

bis(prop-2-en-1-yl) carbonate

Details on test material:

- Name of test material (as cited in study report): DAC
- Physical state: colourless liquid
- Lot/batch No.: 011009
- Expiration date of the lot/batch: October 9, 2002
- Storage condition of test material: ambient conditions
- Other: RTC reference number 5979

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Test item concentration mg/L	Observed immobilisation / total number of animals
	24 hours	48 hours
0	0/10	0/10
1	5/10	5/10
10	10/10	10/10
100	10/10	10/10

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The results obtained indicate that the median lethal concentration (LC 50) to be in the region of 1 mg/L. European directives concerning the classification, packaging and labelling of dangerous substances would suggest the following:
Classification: required
Symbol: N
R-phrase: R50 - very toxic to aquatic organisms