Registration Dossier

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Noakes and Sanderson, Brit. J. industr. Med. 26, 1969, 59
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
p-Chlorobenzyl chloride, solid.

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Average weight at study initiation: female: 167 g, male: 173 g
- Housing: Makrolon cages typ III
- Diet: "Altromin R 1324"
- Water: tap water (ad libitum)
ENVIRONMENTAL CONDITIONS
- Temperature: 22 +/- 1.5 °C
- Humidity: 60 +/- 5 %
- Photoperiod: 12 h dark, 12 h light

Administration / exposure

Type of coverage:
not specified
Vehicle:
polyethylene glycol
Details on dermal exposure:
Amount applied: in polyethylene glycol, once administered
Doses:
1000, 1100, 1200, 1400, 1500, 1600 mg/kg bw
No. of animals per sex per dose:
5 males, 5 females
Control animals:
no
Details on study design:
- Duration of observation period: 14 days

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 287 mg/kg bw
Based on:
test mat.
Gross pathology:
Necropsy of dead and of surviving animals showed no signs of pathological changes.

Applicant's summary and conclusion

Executive summary:

In this acute dermal toxicity study Wistar rats were dermally exposed to p-chlorobenzyl chloride in polyethylen glycol at doses of 1000 mg/kg bw (5/sex), 1100 mg/kg bw (5/sex), 1200 mg/kg bw (5/sex), 1400 mg/kg bw (5/sex), 1500 mg/kg bw (5/sex) and 1600 mg/kg bw (5/sex).

Animals then were observed for 14 days.

Necropsy of dead and of surviving animals showed no signs of pathological changes.

The LD 50 value was established to be 1287 mg/kg bw for both sexes.