Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16.10.1981 - 20.11.1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented study according to in-house method.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981

Materials and methods

Principles of method if other than guideline:
The fish were placed to the aquaria 96 hours before the test. The test substance was dissolved in water and was added to the test water. Duration of the test was 96 h. 10 fishes were exposed to 0; 1; 1.25; 1.6; 2; 2.24; 2.5; 4; 6, 3; 10 and 100 mg/L of 4-chlorobenzyl chloride. Water quality parameters (pH, temperature, oxygen content) were determined at time points 0, 2, 24, 72, and 96 hours to be within the acceptable limits.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Assay:
99.5 % p-Chlorobenzyl chloride
0 -0.5 % p-Chlorobenzal chloride
0 -0.5 % p-Chlorobenzotrichloride
Appearance: Colourless liquid

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
Leuciscus idus f. melanotus
Source: Paul Eggers, Hohenwestedt
Weight: 1.4 - 2.6 g
Length: 5,4 - 6,7 cm

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
48 h and 96 h

Test conditions

Hardness:
192 mg/L NaHCO3
120 mg/L CaSO4 ·H2O
120 mg/L MgSO4
Test temperature:
19 -21 °C
pH:
pH 8.1 - 8.4
Dissolved oxygen:
> 8 mg/L
Salinity:
8 mg KCl
Nominal and measured concentrations:
0 mg/L, 1 mg/L, 1.25 mg/L, 1.6 mg/L, 2 mg/L, 2.24 mg/L, 2.5 mg/L, 4 mg/L, 6.3 mg/L, 10 mg/L, 100 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
2.24 - 2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.24 - 2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.

Applicant's summary and conclusion

Conclusions:
From this study, LC50 for p-chlorobenzyl chloride was determined to be 2.24 - 2.5 mg/L.
Executive summary:

Testing laboratory was Deptm. Pharma Forschung, Toxikologie at Hoechst AG, Frankfurt/Main. A static test with nominal dissolved test substance concentrations was conducted. Duration of the test was 96 h. 10 test organisms were exposed to 0; 1; 1.25; 1.6; 2; 2.24; 2.5; 4; 6.3; 10 and 100 mg/L of 4-chlorobenzyl chloride. Water quality parameters (pH, temperature, oxygen content) were determined at time points 0, 2, 24, 72, and 96 hours to be within the acceptable limits. No lethality occurred in the control group.

At a concentration up to 1.6 mg/L no behavoural abnormalities were observed, no lethality occured till the end of the test.

The highest examined concentration without lethality is 2.24 mg/L after 48 h and 2 mg/L after 96 h.

The lowest examined concentration with 100 % lethality is 2.5 mg/L (after 48 h and 96 h).

As a result, the LC50 value is 2.24 -2.5 mg/L.