Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-493-5 | CAS number: 121-69-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals
- Author:
- Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E.
- Year:
- 1 986
- Bibliographic source:
- Environmental Mutagenesis; Volume 8, Issue Supplement S7, pages 1–55, 1986
- Reference Type:
- other: Authoritative data base
- Title:
- NTP No.: 840758
- Author:
- National Toxicological Program
- Year:
- 2 012
- Bibliographic source:
- NTP (National Toxicological Program)by Agency for Toxic Substances and Disease Registry; Division of Toxicology/Toxicology Information Branch, 1600 Clifton Road NE, E-29, Atlanta, Georgia 30333
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- The Salmonella Ames Test: Preincubation method
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N,N-dimethylaniline
- EC Number:
- 204-493-5
- EC Name:
- N,N-dimethylaniline
- Cas Number:
- 121-69-7
- Molecular formula:
- C8H11N
- IUPAC Name:
- N,N-dimethylaniline
- Details on test material:
- - Name of test material: N,N-dimethylaniline
- Substance type: Organic
- Physical state: liquid
Constituent 1
Method
- Target gene:
- histidine-manufacturing gene
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- other: histidine deficient strain
- Species / strain / cell type:
- S. typhimurium TA 1535
- Additional strain / cell type characteristics:
- other: histidine deficient strain
- Metabolic activation:
- with and without
- Metabolic activation system:
- 10% HLI/RLI
- Test concentrations with justification for top dose:
- 3, 10, 33, 100, 333, 1000 µg/Plate
- Vehicle / solvent:
- Dimethyl Sulfoxide
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl Sulfoxide
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- in the absence of S9 activation
- Positive control substance:
- sodium azide
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl Sulfoxide
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- in the presence of S9 activation by 10% HLI/RLI
- Positive control substance:
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Strain: TA100
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
85 |
2.7 |
93 |
3.7 |
123 |
10 |
87 |
3.1 |
97 |
2.2 |
94 |
9 |
3 |
107 |
3.2 |
107 |
8.5 |
|
|
104 |
4.7 |
|
|
108 |
11.7 |
10 |
89 |
5.6 |
102 |
8 |
112 |
8.7 |
89 |
6 |
110 |
7.9 |
108 |
4.8 |
33 |
104 |
11.3 |
97 |
10.3 |
113 |
1.8 |
104 |
3 |
110 |
6.2 |
101 |
6.2 |
100 |
109 |
12.5 |
95 |
9.9 |
102 |
9.3 |
87 |
3.3 |
113 |
4.5 |
105 |
2.6 |
333 |
102 |
3.5 |
81 |
3.7 |
122 |
12.7 |
93 |
2.7 |
119 |
11.1 |
102 |
8.1 |
1000 |
|
|
|
|
40s |
9.3 |
|
|
78s |
13.4 |
|
|
Positive Control |
382 |
7.7 |
345 |
17.7 |
1336 |
56.6 |
1132 |
40.3 |
461 |
26.6 |
441 |
9.1 |
Strain: TA1535
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
23 |
1.9 |
28 |
3.7 |
9 |
1.9 |
6 |
0.3 |
8 |
0.6 |
10 |
1.2 |
3 |
14 |
0.9 |
20 |
3.8 |
|
|
7 |
1 |
|
|
9 |
0.3 |
10 |
14 |
3.2 |
21 |
2.2 |
10 |
1.2 |
7 |
2.8 |
7 |
0.7 |
8 |
2.2 |
33 |
16 |
1.5 |
18 |
1.9 |
8 |
0.3 |
4 |
1.2 |
6 |
0 |
7 |
0.7 |
100 |
15 |
0.3 |
18 |
2.8 |
6 |
0.3 |
7 |
0.6 |
7 |
0.9 |
6 |
1 |
333 |
15 |
2.1 |
20 |
2.9 |
7 |
1.2 |
6 |
1 |
6 |
1 |
5 |
0.9 |
1000 |
|
|
|
|
2s |
1 |
|
|
3s |
1.2 |
|
|
Positive Control |
519 |
6.8 |
324 |
21.2 |
438 |
43 |
452 |
9.8 |
158 |
11.5 |
187 |
9.9 |
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; T = Toxic; c = Contamination
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
N,N-dimethylaniline gives negative result for genetic toxicity in Ames test conducted on to S. typhimurium TA 100 and TA1535 with and without metabolic activation by 10% HLI/RLI S9 system. - Executive summary:
N,N-dimethylaniline gives negative result for genetic toxicity in Ames test conducted on to S. typhimurium TA 100 and TA1535 with and without metabolic activation by 10% HLI/RLI S9 system.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.