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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
2-Chlorobenzylmercapturic Acid, A Metabolite of the Riot Control Agent 2-Chlorobenzylidene Malononitrile (CS) in the Rat
Author:
E. C. Rietveld x, L. P. C. Delbressine, T. H. J. M.Waegemaekers, and F. Seutter-Berlage
Year:
1983
Bibliographic source:
Arch Toxicol (1983) 54:139-144
Reference Type:
review article or handbook
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Principles of method if other than guideline:
The Salmonella/microsome assay for mutagenicity was performed with salmonella Typhimurium TA 100, according to Ames et al. (1975).
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent

Method

Target gene:
Not specified
Species / strain
Species / strain / cell type:
S. typhimurium TA 100
Test concentrations with justification for top dose:
Test without metabolic activation : 5 concentrations : between 0 to 2000 µg/plate
Test with metabolic activation : several concentrations between 0 to 3500 µg/plate
Vehicle / solvent:
Not specified
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
yes
Remarks:
sodium azide : 1.0 µg or 2.5 µg/plate - benzoapyrène : 5.0 µg/plate
Positive control substance:
sodium azide
benzo(a)pyrene
Details on test system and experimental conditions:
The assays were performed without pre-incubation.

Rationale for test conditions:
Not specified
Evaluation criteria:
Not specified
Statistics:
Not specified

Results and discussion

Test results
Key result
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
1000 µg/plate
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
With rat liver homogenate the bacteriotoxicity of CS was slightly stronger than without it.

Applicant's summary and conclusion

Conclusions:
No mutagenic effect was detected using CS either with or without rat liver homogenate (S-9 mix)
Executive summary:

In a study which methodology is comparable to OECD 471 (Ames test), no mutagenic effect was detected using CS either with or without rat liver homogenate (S-9 mix)