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Diss Factsheets
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EC number: 203-291-4 | CAS number: 105-37-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- in 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: The test was conducted in line with the provisional procedure "Extended toxicity test using Daphnia magna" proposed by the Federal Environmental Agency, Germany.
- Version / remarks:
- 1984
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations, blank preparations (without test organisms or feed)
- Sampling method: samples were taken twice from selected concentration levels of the test series during the test period and analysed chemically. The first sampling was on one of the transfer days before day 7 (period when no offspring appeared). The second sampling was between day 16 and 21. The concentrations were determined in the freshly prepared solutions and in old test solutions after 48 h or 72 h, respectively. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test substance in the stock solution was fully dissolved quantitatively and optically by stirring with magnets. Then the test solutions were prprepared by diluting the stock solution in 1:2 dilution steps with standardised artificial medium (DIN 38412).
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no, test substance optically dissolved - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: IRCHA
- Source: own (maintained since 1978)
- Age of parental stock (mean and range, SD): 24 h-old
- Feeding during test
- Food type: Tetramin (fish feed) and activated sludge
- Amount: 15 - 20 mg COD/L
- Frequency: daily
ACCLIMATION
- Acclimation period: 24 h
- Type and amount of food: green algae Scenedesmus sp., 2 mL of a 9 g dry algae/L suspension
- Feeding frequency: daily - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 2.5 mmol/L (Ca/Mg in dilution water)
- Test temperature:
- 25 +- 1°C
- pH:
- 7 - 8.0
- Dissolved oxygen:
- 58 - 69%
- Nominal and measured concentrations:
- Nominal: 0 (control), 0.8, 1.6, 3.1, 6.3, 12.5, 25, 50 mg/L
Measured: 1.3 mg/L as minimum measured concentration is equivalent to 6.3 mg/L nominal concentration - Details on test conditions:
- TEST SYSTEM
- Test vessel: wide-neck bottles
- Type: closed
- Material, size, headspace, fill volume: glass, ground-glass stoppers
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): 3 times/week
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 animal/50 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 90% deionized water, 10% tap water
- Na/K ratio: 10:1
- Culture medium different from test medium: yes, culture medium: aerated tap water, test medium: artificial medium according to DIN 38412
- Intervals of water quality measurement: at each transfer day
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 9 h light : 15 h dark
- Light intensity: fluorescent lamps with 40/25 W
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : parent mortality, reproduction, appearance of first offspring
RANGE-FINDING STUDY
- Test concentrations: same test concentrations, performed for 3 days
- Results used to determine the conditions for the definitive study: yes, dilution steps have been derived from a preliminary 24 h acute toxicity test (24 h-EC50: 286 mg/L). - Reference substance (positive control):
- yes
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: The most sensitive endpoint was parental mortality beside the reproduction and the number of offspring
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: The most sensitive endpoint was parental mortality beside the reproduction and the number of offspring
- Details on results:
- - Mortality of parent animals: 9.1% (control), most sensitive parameter
- No. of offspring produced per day per female: 68.0 (control, SD: 10.6%, coefficient of variation: 15.6%)
- Time to first brood release: 7 days (control)
- Effect concentrations exceeding solubility of substance in test medium: no, optically clear test solutions - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: The 24 h-EC50 was determined at 0.35 mg/L which indicates a normal toxicity response of the test organisms
- Other: 73 organic and inorganic substances were tested. No explicit positive control was used. Potassium dichromate was tested in a 24 h acute toxicity test which is the positive control in acute toxicity tests (OECD 202). - Validity criteria fulfilled:
- yes
- Conclusions:
- The long-term toxicity of the test substance on Daphnia magna was determined according to the provisional procedure "Extended toxicity test using Daphnia magna" (1984) proposed by the Federal Environmental Agency, Germany. The 21 d-NOEC value for parental mortality as the most sensitive parameter obtained for the test substance was 6.3 mg/L (nominal) and 1.3 mg/L (minimum measured). For further assessments the NOEC value of 1.3 mg/L is taken as key result because the deviation between measured and nominal concentration was more than 20%.
Reference
Table 1: Long-term toxicity results with Ethyl propionate
Chronic toxicity test with D. magna |
|
21 d - NOEC (nominal) |
21 d - NOEC (minimum measured) |
6.3 mg/L |
1.3 mg/L |
The results are based on 7 test concentrations each with 4 replicates. Only the most sensitive endpoint was stated for chronic toxicity (parental animal mortality). The nominal concentrations were analytically verified.
Description of key information
21 d - NOEC = 1.3 mg/L (minimum measured)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.3 mg/L
Additional information
The long-term toxicity of the test substance on Daphnia magna was determined according to the provisional procedure "Extended toxicity test using Daphnia magna" (1984) proposed by the Federal Environmental Agency, Germany. Individuals were exposed to the test substance at 0.8, 1.6, 3.1, 6.3, 12.5, 25, 50 mg/L, which were analytically verified, for 21 days. No further details were reported about the analytical methods used. The NOEC value for parental mortality as the most sensitive parameter obtained for the test substance was 6.3 mg/L (nominal) and 1.3 mg/L (minimum measured). For further assessments the NOEC value of 1.3 mg/L is taken as key reult because the deviation between measured and nominal concentration was more than 20%.
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