Tetrachloro-p-benzoquinone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 08 - August 03, 1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study but no GLP

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1979

Reference 2

Reference Type:

review article or handbook

Title:

BUA Report 85, Ref. Hoechst, 1979

Author:

Beratergremium für umweltrelevante Altstoffe der Gesellschaft Deutscher Chemiker (BUA)

Year:

1992

Bibliographic source:

Hirzel Verlag, Stuttgart

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachloro-p-benzoquinone

EC Number:

204-274-4

EC Name:

Tetrachloro-p-benzoquinone

Cas Number:

118-75-2

Molecular formula:

C6Cl4O2

IUPAC Name:

2,3,5,6-tetrachloro-1,4-benzoquinone

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

6.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Symptoms: several toxic effects (apathy, accelerated respiration, swimming along the water surface, supine position, and anesthesia) were observed at test concentrations above 1.6 mg/L. At 6.3 mg/L all fish died within the first 48 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Remarks:

The following validity criteria from OECD 203 are fulfilled: the mortality in the control did not exceed 10% at the end of the test; the dissolved oxygen concentration was at least 60% of the air saturation value throughout the test.

Conclusions:

The 96h-LC50 determined in this study was 4.6 mg/L.

Executive summary:

In a 96-h acute toxicity study, golden orfe (Leuciscus idus melanotus) were exposed to 3-Hydroxy-2-naphthoic acid at nominal concentrations of 0 (control), Chloranil (2,3,5,6-tetrachloro-1,4-benzoquinone)at nominal concentrations of 0 (control), 1.6, 2.5, 4.0, and 6.3 mg/L under static conditions. No guideline was followed but the principle of the method was equivalent to OECD guideline 203. The study was not performed in compliance with GLP. Reliability grade 2 was assigned.The 96-h LC50 was 4.6 mg/L.Several toxic effects (apathy, accelerated respiration, swimming along the water surface, supine position, and anesthesia) were observed at test concentrations above 1.6 mg/L. At 6.3 mg/L all fish died within the first 48 hours.