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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-06-25 to 1990-11-02
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
- Name of test material (as cited in study report): Sodium Molybdate
- Physical state: white crystalline powder
- Analytical purity: 46.03% Molybdenum, 18.69% sodium
- Purity test date: 1990-06-06
- Storage condition of test material: at room temperature

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: A. Smtith, Warlingham, Surrey
- Age at study initiation: 9 to 11 weeks
- Weight at study initiation: 2.2 to 2.6 kg
- Housing: individually in metal cages with perforated floors
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 °C
- Humidity (%): 30 to 70 %
- Air changes (per hr): 19
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:
semiocclusive
Preparation of test site:
clipped
Vehicle:
water
Controls:
no
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 g of Sodium molybdate moistened with 0.5 mL of distilled water
Duration of treatment / exposure:
4 hours
Observation period:
4 days
Number of animals:
6 females
Details on study design:
TEST SITE
- Area of exposure: 2.5 cm²
- Type of wrap if used: elastic adhesive dressing "Elastoplast"

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with water
- Time after start of exposure: 4 hours

SCORING SYSTEM:
Erythema and eschar formation:
0 - No erythema
1 - Very slight erythema (barely perceptible)
2 - Well-defined erythema
3 - Moderate to severe erythema
4 - Severe erythema (beet redness) to slight eschar formation (injuries in depth)

Oedema formation:
0 - No oedema
1 - Very slight oedema (barely perceptible)
2 - Slight oedema (edges of area well-defined by definite raising)
3 - Moderate oedema (raised approximately 1 millimetre)
4 - Severe oedema (raised more than 1 millimetre and extending beyond the area of exposure)

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 0.5 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 0.5 h
Score:
0
Max. score:
0
Irritation parameter:
erythema score
Basis:
mean
Time point:
24 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
mean
Time point:
24 h
Score:
0
Max. score:
0
Irritation parameter:
erythema score
Basis:
mean
Time point:
48 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
mean
Time point:
48 h
Score:
0
Max. score:
0
Irritation parameter:
erythema score
Basis:
mean
Time point:
72 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
mean
Time point:
72 h
Score:
0
Max. score:
0
Irritant / corrosive response data:
No irritation
Other effects:
None

Any other information on results incl. tables

Table 1: Dermal reactions elicited by Sodium Molybdate
Rabbit number*  E = Erythema Day
O = Oedema 1** 2 3 4
 
2481 E 0 0 0 0
O 0 0 0 0
2482 E 0 0 0 0
O 0 0 0 0
2483 E 0 0 0 0
O 0 0 0 0
2484 E 0 0 0 0
O 0 0 0 0
2485 E 0 0 0 0
O 0 0 0 0
2486 E 0 0 0 0
O 0 0 0 0

*= only female animals were tested

**= approximately 30 minutes after removal of dressing

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
In conclusion, in an acute dermal irritation/corrosion study according to OECD 404, the read-across partner Sodium molybdate was found to be non-irritating.
Executive summary:

In a primary dermal irritation study (OECD guideline 404) six female New Zealand white rabbits were dermally exposed to 0.5 g of Sodium molybdate for 4 hours to a body surface area of 2.5 cm² under semi-occlusive conditions. Animals then were observed for 0.5, 24, 48 and 72 hours. Sodium molybdate did not cause any skin effects and is considered under the conditions of this study as non toxic.