2-Naphthalenesulfonic acid, 4-hydroxy-3-[2-[2-methoxy-5-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-7-[(sulfomethyl)amino]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, sodium salt (1:5)

Short-term toxicity to aquatic invertebrates

Description of key information

The 48 h EC0 (and thus EC50) of the test substance was ≥ 100 mg/L (equivalent to 101.3 mg/L mean measured concentration).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

An acute immobilization test was performed to evaluate the toxicity of the test substance toDaphnia magnaaccording to EU Method C.2, which is equivalent to OECD guideline 202, in compliance with GLP. In this test, 10 daphnids per vessel were exposed to the test substance at a nominal concentration of 100 mg/L under static conditions for 48 h. Immobilisation rates were recorded at 24 h intervals. A temperature range of 18 -22°C was maintained in the test vessels, with a maximum temperature fluctuation of ±1°C. pH and oxygen values were measured at the end of the study. Doses were verified analytically with HPLC. No immobilization was observed in control as well as test concentration groups. Measured concentrations corresponded to 99.4% of nominal values at 0 h, and to 101.3% of nominal values at 48 h, respectively. Results were therefore expressed based on nominal value. Under the conditions of the study, the 48 h EC0 (and thus EC50) of the test substance was ≥ 100 mg/L (equivalent to 101.3 mg/L as mean measured concentration) (Bruns, 2005b).