Solsperse 22000-Wirksubstanz

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

2001

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

2001

Deviations:

no

Principles of method if other than guideline:

Deviation from the guideline: The log Koc of 2-nitrobenzamide was taken from mean value of batch experiments on five soils adopted from GAVLIK & MUNTAU (1999):
A different batch of Atrazine was used. The expiry date of Triademinol was corrected.

These deviations were considered to have no impact on quality and integrity of the study.

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

sewage sludge

Test material

Test material information

Constituent 1

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Specific details on test material used for the study:

solubility in water: <1 mg/L (room temperature)
pH-value: 6 - 7 (suspension)
melting point >230°C
content: 98.3% (w/w) organic component

Radiolabelling:

no

Study design

Test temperature:

23°C

HPLC method

Details on study design: HPLC method:

Injection volume: 50.0 µL
Mobile phase: Tetrahydrofurane : water / 55 :45 (v/v)
Column temperature: 23°C
Flow: 0.50 mL/min
Detector: Diode array Detector (DAD)
Wavelenght: 200 - 500 nm (220 for evaluation of reference items and 425 for test item)
Run time: 10 min

Calibration: The method was calibrated by determination of the retention times t(r) of six reference items. Each reference item was analysed separately before and after the analysis of the test item. Analyses were checked for constant retention times during the measurements. A calibration graph of log k' versus log Koc was established to correlate the measured HPLC-retention data of the test item with adsorption coefficient Koc.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

At test conditions two peaks for the test item Solsperse 22000 were found at 1.629 and 8.036 min which could be clearlöy classified as belonging to the test item due to their UV spectrum, showing maxima at 264 and 425 nm. The first peak eluted before dead time of sodium nitrate and the second one after 4,4'-DDT which has the higest logKoc of the reference items established. Therefore both peaks were not evalueble and calculation of logKoc can not be performed.

Executive summary:

At test conditions two peaks for the test item Solsperse 22000 were found at 1.629 and 8.036 min which could be clearlöy classified as belonging to the test item due to their UV spectrum, showing maxima at 264 and 425 nm. The first peak eluted before dead time of sodium nitrate and the second one after 4,4'-DDT which has the higest logKoc of the reference items established. Therefore both peaks were not evalueble and calculation of logKoc can not be performed.