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Short-term toxicity to aquatic invertebrates

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Description of key information

Daphnia sp., Acute Immobilization Test was performed according to OECD Guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media (prepared in MilliQ water). The test chemical was exposed to daphnia at a limit concentration of 100 mg/L for a period of 48 hours in a static condition.

A 25 mL of glass beaker was used to perform the experiment which was filled with 20mL of media including the test chemical.

The end point to determine the EC50 concentration was the immobilization of the test species. Median Effect Concentration (EC50) of test chemical on Daphnia magna was determined as >100 mg/L. Based on the EC50 value it can be consider that the test chemical is nontoxic and can be classified under the category of “not classified chemical” as per the CLP classification criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

Following experimental studies for the test chemical was summarized to conclude the toxicity extent of test substance towards aquatic invertebrate is as follows:

In the first experimental key study

Daphnia sp., Acute Immobilization Test was performed according to OECD Guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media (prepared in MilliQ water). The test chemical was exposed to daphnia at a limit concentration of 100 mg/L for a period of 48 hours in a static condition.

A 25 mL of glass beaker was used to perform the experiment which was filled with 20mL of media including the test chemical.

The end point to determine the EC50 concentration was the immobilization of the test species. Median Effect Concentration (EC50) of test chemical on Daphnia magna was determined as >100 mg/L. Based on the EC50 value it can be consider that the test chemical is nontoxic and can be classified under the category of “not classified chemical” as per the CLP classification criteria.

First study was supported by the second from peer reviewed journal. Short term toxicty test was carried out according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) with Daphnia magna as a test organisms.

The effective concentration (EC10) value of test chemical in Daphnia magna in a 24 hr study on mortality effect was found to be 304 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it is concluded that test substancedoes not exhibit short term toxicity to aquatic invertebrate (Daphnia magna).

Similar study from the other peer reviewed journal also suggests that To study the toxic effects of test chemical on aquatic invertebrate, short term toxicity test was carried out for 48 hr using daphnia magna asa test organism.

Lethal Threshold Concentration value of test chemical in Daphnia magna in a 48 hr study on intoxication effect was found to be 1450 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it is concluded that test sbstance does not exhibit short term toxicity to aquatic invertebrate (Daphnia magna).

Thus based on the overall studies chemical concluded to be nontoxic and not classified as per the CLP classification criteria.