Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Study was conducted to access the effect of test chemical on the growth of zebra fishDanio rerio. Test was performed according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring.

The zebrafishes were exposed to the concentration of 100mg/ L, and test was conducted for the period of 96 hrs. the exposed fishes were observed for any visual behavioural abnormality and mortality.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used and the study was conducted in compliance with the OECD guideline 203. After the 96 hours of exposure to test item at nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Following experimental studies for the test chemical was summarized to conclude the extent of toxicity of test substance in fishes is as follows:

 

Study was conducted to access the effect of test chemical on the growth of zebra fishDanio rerio. Test was performed according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring.

The zebrafishes were exposed to the concentration of 100mg/ L, and test was conducted for the period of 96 hrs. the exposed fishes were observed for any visual behavioural abnormality and mortality.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used and the study was conducted in compliance with the OECD guideline 203. After the 96 hours of exposure to test item at nominal test concentrations, LC50 was determine to be >100 mg/l .

 

Key study was supported by publication from the peer reviewed journal, Acute Toxic studies of the test chemical was studied on the fresh water fishPimephales promelas. The test was performed for the period of 96 hrs, based on the mortality of the fishes, the median lethal concentration was determined. Stocks were prepared in 18.9 L stainless steel carbonation vessels, by mixing test substance in the Lake Superior water. The median lethal concentration of test chemical (LC50) was determined to be 1370 mg/L. Most of the effected fishes at concentration greater than 1370 have lost their schooling behaviour and were swimming at the surface.

 

Key study was supported by publication from peer revied journal, Test chemical was evaluated for the determination of median lethal concentration (LC50) using fish as a animal model, for the tenure of 96 h. Test chemical was exposed to the nominal concentrations, ranging from 0 to 3080 with the geometric mean of 1.53. The experiment was conducted in the flow through condition.

The basis for the determination of the LC50 concentration was mortality. Most of the fishes lost their schooling behaviour, equilibrium and were hyperactive prior to their death. The median lethal concentration of the fish was determined as 2160 mg/L.

Based on the overall availale study report and publication data, the test chemical cannot be classified.