Dimethyl 3-oxoglutarate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl 3-oxoglutarate

EC Number:

217-385-8

EC Name:

Dimethyl 3-oxoglutarate

Cas Number:

1830-54-2

Molecular formula:

C7H10O5

IUPAC Name:

dimethyl 3-oxoglutarate

Details on test material:

- Name of test material (as cited in study report): LZ 409
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance):
- Smiles notation (if other than submission substance):
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: clear yellow liquid
- Analytical purity:
- Impurities (identity and concentrations):
- Composition of test material, percentage of components:
- Isomers composition:
- Purity test date:
- Lot/batch No.:
- Expiration date of the lot/batch:
- Radiochemical purity (if radiolabelling):
- Specific activity (if radiolabelling):
- Locations of the label (if radiolabelling):
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions:
- Storage condition of test material: Store at room temparature, in the dark
- Other:

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
The reference material: sodium-benzoate (Batch 10K0179) was introduced to the test system at 2 mg/L.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Obtained from Haddington Municipal Sewage Treatment Works
- Laboratory culture:
- Method of cultivation:
- Storage conditions:
- Storage length:
- Preparation of inoculum for exposure:
- Pretreatment:
- Concentration of sludge: Solid content of the sludge was determinded as 4.2 g/L.
- Initial cell/biomass concentration:
- Water filtered: yes/no
- Type and size of filter used, if any:

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

4.65 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

2 mg/L

Based on:

other: sodium-benzoate

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Duplication bottles for control, test and reference material were prepared using a sewage sludge inoculum and were sampled for dissolved oxygene concentration on days 0, 5, 7, 10, 14, 21, and 28 to determine oxygene depletion. This was then related to ThOD and concentration of test item or reference material and expressed as per cent bidegradation.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

13.67

Sampling time:

5 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

62.35

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

69.35

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Under test conditions the test item and the reference material were readily biodegradable .
The test item did not appear to exhibit any inhibitory effects on the microbial inoculum.