1,2-dichloro-3-nitrobenzene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Version / remarks:

adopted in 1981

Deviations:

yes

Remarks:

observation period 8 d

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,2-dichloro-3-nitrobenzene

EC Number:

221-717-7

EC Name:

1,2-dichloro-3-nitrobenzene

Cas Number:

3209-22-1

Molecular formula:

C6H3Cl2NO2

IUPAC Name:

1,2-dichloro-3-nitrobenzene

Details on test material:

- Analytical purity: No data

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Lippische Versuchstierzucht, Hagemann GmbH & Co. KG, Extertal, Germany
- Weight at study initiation: 2.7 kg
- Housing: single
- Diet (e.g. ad libitum): mümmel z (ssniff, Soest, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18
- Humidity (%): 40
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 µl bulk volume

Duration of treatment / exposure:

continuous

Observation period (in vivo):

1, 24, 48, and 72 h, and 8 days

Number of animals or in vitro replicates:

3

Details on study design:

TOOL USED TO ASSESS SCORE: fluorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

2

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

3

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Results 5

Irritation parameter:

conjunctivae score

Remarks:

redness

Basis:

mean

Time point:

other: 1 h

Score:

1

Max. score:

3

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: fully reversible

Results 6

Irritation parameter:

conjunctivae score

Remarks:

swelling

Basis:

mean

Time point:

other: 1 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: fully reversible

Results 7

Irritation parameter:

conjunctivae score

Remarks:

efflux

Basis:

mean

Time point:

other: 1 h

Score:

2

Max. score:

3

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: fully reversible

Results 8

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 1, 24, 48 and 72 h

Score:

0

Max. score:

4

Reversibility:

other: no effects

Remarks on result:

other: no effects

Results 9

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 1, 24, 48 and 72 h

Score:

0

Max. score:

2

Reversibility:

other: no effects

Remarks on result:

other: no effects

Any other information on results incl. tables

No signs of eye irritation according to cornea and iris scores were observed throughout the test and observation period. One hour after application of the test substance, all animals showed slight conjunctival redness and swelling, as well as moderate efflux. These effects were fully reversible within 24 h.

Applicant's summary and conclusion

Executive summary:

The eye irritation potential of 1,2-dichloro-3-nitrobenzene was investigated in 3 New Zealand White rabbits compliant with the OECD guideline 405 (adopted: 12 May 1981). A bulk volume of 0.1 mL of the crystalline test substance was applied into the conjunctival sac of the right eye of each animal, the left eye serving as control. The eyes were not rinsed after administration of the test substance. The eye irritation reactions were examined and scored using the Draize method 1, 24, 48 and 72 h as well as 8 days after application. 1,2-dichloro-3-nitrobenzene induced slight conjunctival redness and swelling, as well as moderate efflux 1 h after administration. These effects were fully reversible within 24 h. The mean scores over 24, 48 and 72 h were 0.0 for corneal opacity and iritis in all animals (Schreiber, 1982).