Registration Dossier
Registration Dossier
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EC number: 221-717-7 | CAS number: 3209-22-1
Specific investigations: other studies
Administrative data
- Endpoint:
- nephrotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well-documented publication
Data source
Reference
- Reference Type:
- publication
- Title:
- In vitro nephrotoxicity induced by chloronitrobenzenes in renal cortical slices from Fischer 344 rats
- Author:
- Hong, S.K. et al.
- Year:
- 2 002
- Bibliographic source:
- Toxicology Letters 129: 133-141
Materials and methods
- Principles of method if other than guideline:
- Determination of gluconeogenesis capacity and lactate dehydrogenase release in renal cortical slices as measures of cellular function and cytotoxicity for the assessment of direct nephrotoxic effects.
- GLP compliance:
- not specified
- Type of method:
- in vitro
- Endpoint addressed:
- not applicable
Test material
- Reference substance name:
- 1,2-dichloro-3-nitrobenzene
- EC Number:
- 221-717-7
- EC Name:
- 1,2-dichloro-3-nitrobenzene
- Cas Number:
- 3209-22-1
- Molecular formula:
- C6H3Cl2NO2
- IUPAC Name:
- 1,2-dichloro-3-nitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2,3-dichloronitrobenzene
- Analytical purity: no data
- Source: Aldrich Chemical Co. (Milwaukee, WI)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hilltop Lab Animals, Inc. (Scottdale, PA)
- Weight at study initiation: 200-250 g
- Housing: standard plastic animal cages
- Diet (e.g. ad libitum): Purina Rat Chow ad libitum
- Water (e.g. ad libitum):ad libitum
- Acclimation period: at least 1 week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23
- Humidity (%): 40-55
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- other: not applicable, in vitro study
Results and discussion
- Details on results:
- 2,3-Dichloronitrobenzene was effective at decreasing pyruvate-stimulated gluconeogenesis at bath concentrations of 0.5 mM or greater. A bath concentration of 1.0 mM or greater was required for 2,3-dichloronitrobenzene to increase LDH realease.
Applicant's summary and conclusion
- Executive summary:
The results of this study demonstrate that chloronitrobenzenes are directly toxic to the kidney in a concentration-dependent manner. As expected, disruption of cellular function (decreased pyruvate-stimulated gluconeogenesis) normally
occurred at lower bath concentrations of the chloronitrobenzenes than the appearance of overt cytotoxicity (increased LDH release). Based on the toxic effects induced by the chloronitrobenzenes, the decreasing order of nephrotoxic potential to rat renal cortical slices was trichloronitrobenzenes > dichloronitro- benzenes > monochloronitrobenzenes > nitrobenzene.
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