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EC number: 270-109-8 | CAS number: 68411-20-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Direct weighings were prepared to give the different test item concentrations. The test item was added into Erlenmeyer flasks (incubation vessels) to about 130 mL deionised water and was stirred before testing (equilibration phase) overnight for 16 hours.
For the reference compound a stock solution at a concentration of 500 mg/L was prepared by dissolving 250 mg 3,5-Dichlorophenol in 5 mL of 1 N NaOH and diluting to 0.5 litre with deionised water. The pH was adjusted to pH 7 ± 0.5 with HCl. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - mixed population of aquatic microorganisms, origin of an aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim, Germany)
- pretreatment: aeration, daily feed with synthetic medium
- pH 7.4
- test concentration of the activated sludge: 800 mg/L suspended solids
- Preparation of inoculum for exposure:
- first, the sludge was settled and the supernatant was decanted
- after centrifuging (15 min at 4500 rpm and 20°C) the supernatant was decanted
- approx. 1 g of the wet sludge was dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 3 g/L (dry weight) suspended solids
- the calculated amount of sludge was first dissolved in synthetic medium and than filled up to a defined end volume with deionised water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- no data
- Test temperature:
- 20.1 (concerning the highest concentration of 1000 mg/l)
- pH:
- 7.9 (concerning the highest concentration of 1000 mg/l)
- Dissolved oxygen:
- oxygen (mg/l): O2-start 4.8; O2-end 2.5 (concerning the highest concentration of 1000 mg/l)
- Salinity:
- no data
- Nominal and measured concentrations:
- 10, 100, 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
Before use the wet weight/dry weight relationship of the activated sludge was determined by drying 10 mL of sludge suspension. Subsequently, a sludge suspension of 2 g (dry weight)/L was prepared. The pH of this suspension was measured and adjusted to 6-8.
8 mL of the synthetic medium and 100 mL of activated sludge were added to the dissolved test item. The mixture was filled up with deionised water to 250 mL and aerated at 20 ± 2 °C.
The exposure medium with the reference substance was prepared by adding 8 mL of the synthetic medium, 100 mL of activated sludge and a defined amount of the stock solution to achieve the test concentrations, and was filled up with deionised water to 250 mL and aerated at 20 ± 2°C.
Control vessels (inoculated sample without test item) were prepared the same way.
Additional vessels to determine the physico-chemical oxygen consumption were prepared containing the test item, and the synthetic medium but no activated sludge.
- Test vessel: 300 mL glass Erlenmeyer flasks
- type of application: direct weighing
- temperature: 20 +/- 2 °C
- stirring period before start of incubation time: 16 h (equilibration phase)
- test concentration: 3 (10, 100, 1000 mg/L)
- test parameter: respiration rate
- incubationtime: 3 hours with permanent aeration - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol, concentration (5, 10, 20 mg/L) )
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - analysis of the respiration rates: by probit analysis
- Results with reference substance (positive control):
- - EC50 of the reference compound 3,5-Dichlorophenol is in the range 5 – 30 mg/L
- Validity criteria fulfilled:
- yes
- Remarks:
- respiratory rates of the 2 controls differ less than 15 % from each other
- Conclusions:
- According to EU Method C.11, the test item showed 0.0 % respiration inhibition of activated sludge at the highest test item concentration of 1000 mg/L.
- Executive summary:
In order to test the toxicity to microorganism of the substance, activated sludge from predominantly domestic sewage plant
was exposed to the test solution of three nominal concentrations of the substance (10, 100, and 1000 mg/L), blank control
solution and solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and
respiration rates were claculated. No physico-chemical oxygen consumption was observed at the highest concentration of
test substance. No respiration inhibition was observed at 10,100 and 1000 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microoragnisms.
Reference
Description of key information
According to EU Method C.11, the test item showed 0.0 % respiration inhibition of activated sludge at the highest test item concentration of 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
should read >1000 mg/L.
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