Citraconic anhydride

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was performed according to methods similar to OECD 405, not accoring to GLP. Details on test substance and results are limited.

Data source

Reference

Reference Type:

publication

Title:

Studies on eye irritation caused by chemicals in rabbits -1. A quantitative structure-activity relationships approach to primary eye irritation of chemicals in rabbit.

Author:

Sugai, S., Murata, K. Kitagaki, T., Tomita, I.

Year:

1990

Bibliographic source:

The Journal of Toxicological Sciences, Vol. 15, 245-262

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Citraconic anhydride

EC Number:

210-459-0

EC Name:

Citraconic anhydride

Cas Number:

616-02-4

Molecular formula:

C5H4O3

IUPAC Name:

3-methyl-2,5-dihydrofuran-2,5-dione

Details on test material:

Name: citraconic anhydride

Test animals / tissue source

Species:

rabbit

Strain:

other: Japanese white rabbits

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Takeo breeder NII-Electronic Library Service
- Age at study initiation: no data
- Weight at study initiation: 2.0-2.2 kg
- Housing:housed in metal cages (39 X 40 X 44 cm)
- Diet (e.g. ad libitum): fed the RC4 diet (Oriental Yeast Co., Tokyo, Japan), ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period:

ENVIRONMENTAL CONDITIONS
- Temperature (°C):25 ± 1°C
- Humidity (%): 55 ± 5%
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: no data

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The right eye was served as a blank.

Amount / concentration applied:

0.1 ml

Duration of treatment / exposure:

single application

Observation period (in vivo):

The eyes were examined and the grade of ocular reaction was recorded 1, 4, 24, 48, 72, 96hr, 7, 14 and 21 days after the treatment of chemicals.

Number of animals or in vitro replicates:

3 females

Details on study design:

REMOVAL OF TEST SUBSTANCE: none

SCORING SYSTEM: Corneal opacity, erythema, chemosis, secreta and iritis were classified according to the Draize score

TOOL USED TO ASSESS SCORE: no data

Results and discussion

In vivo

Results

Irritation parameter:

other:

Basis:

other: all animals

Time point:

other: up to 21 days

Reversibility:

not reversible

Remarks on result:

other: Score III: Corrosive

Irritant / corrosive response data:

The only data given is that the outcome of the test is corrosive for this test substance. This is confirmed by a QSAR.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye)

Remarks:

Criteria used for interpretation of results: expert judgment

Conclusions:

The test substance is corrosive to the eye according to the Draize scoring method: Corneal involvement, irritation or eye damage that persists for more than 21 days after treatment.

Executive summary:

The test substance is corrosive to the eye according to the Draize scoring method: Corneal involvement, irritation or eye damage that persists for more than 21 days after treatment.